Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Heart
DISEASE(S): Disease Free
SUBMITTER: david greening
LAB HEAD: David Greening
PROVIDER: PXD023570 | Pride | 2021-04-26
REPOSITORIES: Pride
Claridge Bethany B Rai Alin A Fang Haoyun H Matsumoto Aya A Luo Jieting J McMullen Julie R JR Greening David W DW
Proteomics 20210506 13-14
Cardiac intercellular communication is critical for heart function and often dysregulated in cardiovascular diseases. While cardiac extracellular vesicles (cEVs) are emerging mediators of signalling, their isolation remains a technical challenge hindering our understanding of cEV protein composition. Here, we utilised Langendorff-collagenase-based enzymatic perfusion and differential centrifugation to isolate cEVs from mouse heart (yield 3-6 μg/heart). cEVs are ∼200 nm, express classical EV mark ...[more]