Dataset Information

AP-MS of helicase Upf1 in PC12 cells

ABSTRACT: DNA/RNA helicase Upf1 seems to interact with axonal transcripts in response to nerve growth factor (NGF, A. Ludanyi, M. Gaspari and A. Riccio, unpublished data). In order to shed light on Upf1 mechanism of action and to identify potential associations with molecules having cleavage activity, we performed an AP-MS experiment comprising Upf1 immunoprecipitation, on-beads digestion, isotopic labelling (18O) and quantitative LC-MS/MS analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture

SUBMITTER: Marco Gaspari

LAB HEAD: Marco Gaspari

PROVIDER: PXD023586 | Pride | 2021-09-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	UPF1-H_NGF-L_Cont-Rep1a.msf	Msf
	UPF1-H_NGF-L_Cont-Rep1a.raw	Raw
	UPF1-H_NGF-L_Cont-Rep1b.msf	Msf
	UPF1-H_NGF-L_Cont-Rep1b.raw	Raw
	UPF1-H_NGF-L_Cont-Rep2a.msf	Msf

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Publications

Cytoplasmic cleavage of IMPA1 3' UTR is necessary for maintaining axon integrity.

Andreassi Catia C Luisier Raphaëlle R Crerar Hamish H Darsinou Marousa M Blokzijl-Franke Sasja S Lenn Tchern T Luscombe Nicholas M NM Cuda Giovanni G Gaspari Marco M Saiardi Adolfo A Riccio Antonella A

Cell reports 20210201 8

The 3' untranslated regions (3' UTRs) of messenger RNAs (mRNAs) are non-coding sequences involved in many aspects of mRNA metabolism, including intracellular localization and translation. Incorrect processing and delivery of mRNA cause severe developmental defects and have been implicated in many neurological disorders. Here, we use deep sequencing to show that in sympathetic neuron axons, the 3' UTRs of many transcripts undergo cleavage, generating isoforms that express the coding sequence with ...[more]

PMID: 33626357

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