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MicroRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development

ABSTRACT: Proteomic mass spectrometry was used to identify interactors of the miRNRA-Induced Silencing Complex in C. elegans.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: Yasaman Jami-Alahmadi

LAB HEAD: Thomas Duchaine

PROVIDER: PXD023610 | Pride | 2021-09-10

REPOSITORIES: Pride

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			Action	DRS
	2018-02-08-Duchaine-VInay-FD03-NH4OH-1.mzXML	Mzxml
	2018-02-08-Duchaine-VInay-FD03-NH4OH-1.mzid.gz	Mzid
	2018-02-08-Duchaine-VInay-FD03-NH4OH-1.raw	Raw
	2018-02-08-Duchaine-VInay-FD03-NH4OH-2.mzXML	Mzxml
	2018-02-08-Duchaine-VInay-FD03-NH4OH-2.raw	Raw

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Publications

microRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development.

Mayya Vinay K VK Flamand Mathieu N MN Lambert Alice M AM Jafarnejad Seyed Mehdi SM Wohlschlegel James A JA Sonenberg Nahum N Duchaine Thomas F TF

Nucleic acids research 20210501 9

microRNA (miRNA)-mediated gene silencing is enacted through the recruitment of effector proteins that direct translational repression or degradation of mRNA targets, but the relative importance of their activities for animal development remains unknown. Our concerted proteomic surveys identified the uncharacterized GYF-domain encoding protein GYF-1 and its direct interaction with IFE-4, the ortholog of the mammalian translation repressor 4EHP, as key miRNA effector proteins in Caenorhabditis ele ...[more]

PMID: 33758928

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microRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development.

Project description:microRNA (miRNA)-mediated gene silencing is enacted through the recruitment of effector proteins that direct translational repression or degradation of mRNA targets, but the relative importance of their activities for animal development remains unknown. Our concerted proteomic surveys identified the uncharacterized GYF-domain encoding protein GYF-1 and its direct interaction with IFE-4, the ortholog of the mammalian translation repressor 4EHP, as key miRNA effector proteins in Caenorhabditis elegans. Recruitment of GYF-1 protein to mRNA reporters in vitro or in vivo leads to potent translation repression without affecting the poly(A) tail or impinging on mRNA stability. Loss of gyf-1 is synthetic lethal with hypomorphic alleles of embryonic miR-35-42 and larval (L4) let-7 miRNAs, which is phenocopied through engineered mutations in gyf-1 that abolish interaction with IFE-4. GYF-1/4EHP function is cascade-specific, as loss of gyf-1 had no noticeable impact on the functions of other miRNAs, including lin-4 and lsy-6. Overall, our findings reveal the first direct effector of miRNA-mediated translational repression in C. elegans and its physiological importance for the function of several, but likely not all miRNAs.

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