Dataset Information

The injury environment critically influences the brain-wide input connectome of transplanted neurons

ABSTRACT: Cell transplantation is a promising approach for reconstruction of neuronal circuits after brain damage. Transplanted neurons integrate with remarkable specificity into circuitries of the mouse cerebral cortex affected by neuronal ablation. However, it remains unclear how neurons perform in a local environment undergoing reactive gliosis, inflammation, macrophage infiltration and scar formation, as in brain trauma. To elucidate this, we transplanted cells from the embryonic murine cerebral cortex into stab-injured, inflamed-only, or intact cortex of adult mice. Brain-wide quantitative connectomics unraveled graft inputs from correct regions across the brain in all conditions, with pronounced quantitative differences: scarce in intact or inflamed brain, versus exuberant after trauma. In the latter, excessive synapse pruning follows the initial overshoot of connectivity resulting in only a few input connections left. Proteomic profiling identifies candidate molecules involved in the synaptic yield, a pivotal parameter to tailor for functional restoration of neuronal circuits.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Stefanie Hauck

LAB HEAD: Stefanie M. Hauck

PROVIDER: PXD023660 | Pride | 2022-08-12

REPOSITORIES: Pride

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			Action	DRS
	THJ13321X002__WT-3m_rep2_left_92-194133.raw	Raw
	THJ13321X006__WT-3m_intact_R4_92-194137.raw	Raw
	THJ13321X007__WT-3m_SW_L5_ipsi_92-194138.raw	Raw
	THJ13321X008__WT-3m_rep1_ipsi_92-194139.raw	Raw
	THJ13321X016__WT-3m_intact_L2_92-194147.raw	Raw

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Brain injury environment critically influences the connectivity of transplanted neurons.

Grade Sofia S Thomas Judith J Zarb Yvette Y Thorwirth Manja M Conzelmann Karl-Klaus KK Hauck Stefanie M SM Götz Magdalena M

Science advances 20220610 23

Cell transplantation is a promising approach for the reconstruction of neuronal circuits after brain damage. Transplanted neurons integrate with remarkable specificity into circuitries of the mouse cerebral cortex affected by neuronal ablation. However, it remains unclear how neurons perform in a local environment undergoing reactive gliosis, inflammation, macrophage infiltration, and scar formation, as in traumatic brain injury (TBI). To elucidate this, we transplanted cells from the embryonic ...[more]

PMID: 35687687

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