Proteomics

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Proteome analysis of Mycobacterium fortuitum biofilm

ABSTRACT: Biofilm formation by the environmental and clinical contaminant Mycobacterium fortuitum causes economic losses and serious threat to human health, as a consequence of its increasing contribution to nosocomial infections. There are no reports that elucidate physiological adaptations taking place during its planktonic to biofilm transition. The present study was hence carried out considering the global proteome of the mycobacterium. This is the first description of a global proteomic investigation into M. fortuitum biofilm. Scrutiny of biological functions in the two states provided insights into the phenotypic switch, and fundamental pathways associated with M. fortuitum pathobiology.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mycolicibacterium Fortuitum Subsp. Fortuitum Dsm 46621 = Atcc 6841

SUBMITTER: Rahul Shrivastava  

LAB HEAD: Rahul Shrivastava

PROVIDER: PXD023784 | Pride | 2023-09-11

REPOSITORIES: Pride

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Publications

Comparative proteomic investigation unravels the pathobiology of Mycobacterium fortuitum biofilm.

Sharma Ayushi A   Bansal Saurabh S   Kumari Neha N   Vashistt Jitendraa J   Shrivastava Rahul R  

Applied microbiology and biotechnology 20230805 19

Biofilm formation by Mycobacterium fortuitum causes serious threats to human health due to its increased contribution to nosocomial infections. In this study, the first comprehensive global proteome analysis of M. fortuitum was reported under planktonic and biofilm growth states. A label-free Q Exactive Quadrupole-Orbitrap tandem mass spectrometry analysis was performed on the protein lysates. The differentially abundant proteins were functionally characterized and re-annotated using Blast2GO an  ...[more]

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