Dataset Information

Characterisation of a cell-free myxoid liposarcoma patient-derived xenograft scaffold model

ABSTRACT: Myxoid liposarcoma (MLS) is the second most common type of liposarcoma, and today few model systems to study the disease exists. To be able to model the disease in vitro, cell-free scaffolds from MLS patient-derived xenograft (PDX) models were generated. The MLS scaffolds were then used as a 3D growth platform for MLS cell lines to study the cancer microenvironments impact on cellular heterogeneity using RNA sequencing. Key components in the microenvironment have also been shown to influence the fraction of cellular subpopulations, such as cancer stem cells and migratory cells but also to promote aggressive features of cancers. Therefore, to better understand and characterize these scaffolds, protein analyses were performed and links between scaffold compositions and the induced gene expression profiles of the cells grown therein could be made. This model system provides a better insight to the composition of the cell-free cancer microenvironment in a rare disease, which can lead to identification of novel malignancy inducing properties in MLS.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liposarcoma Cell

DISEASE(S): Myxoid Liposarcoma

SUBMITTER: Carina Sihlbom

LAB HEAD: Carina Sihlbom

PROVIDER: PXD023792 | Pride | 2024-05-08

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2876_3sets_0mc_Fusion_180514_32_101.msf	Msf
	2876_NONORM_3sets_0mc_SNquan_CorrFactOn_Fusion_180514_32_101.xlsx	Xlsx
	Fusion_180514_100.raw	Raw
	Fusion_180514_101.raw	Raw
	Fusion_180514_32.raw	Raw

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Publications

Deciphering the role of FUS::DDIT3 expression and tumor microenvironment in myxoid liposarcoma development.

Ranji Parmida P Jonasson Emma E Andersson Lisa L Filges Stefan S Luna Santamaría Manuel M Vannas Christoffer C Dolatabadi Soheila S Gustafsson Anna A Myklebost Ola O Håkansson Joakim J Fagman Henrik H Landberg Göran G Åman Pierre P Ståhlberg Anders A

Journal of translational medicine 20240426 1

<h4>Background</h4>Myxoid liposarcoma (MLS) displays a distinctive tumor microenvironment and is characterized by the FUS::DDIT3 fusion oncogene, however, the precise functional contributions of these two elements remain enigmatic in tumor development.<h4>Methods</h4>To study the cell-free microenvironment in MLS, we developed an experimental model system based on decellularized patient-derived xenograft tumors. We characterized the cell-free scaffold using mass spectrometry. Subsequently, scaff ...[more]

PMID: 38671504

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