Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Exploring the glycosylation state of the Cryptosporidium parvum CpTSP protein family-FAIMS proteome

ABSTRACT: Cryptosporidium parvum is a zoonotic apicomplexan parasite and a common cause of diarrheal disease worldwide. The development of vaccines to prevent or limit infection remains an important goal for tackling these diarrheal diseases, which are a significant cause of infant morbidity in the developing world. The only approved vaccine against an apicomplexan parasite targets conserved adhesins possessing a thrombospondin repeat (TSR) domains. Orthologous TSR domain-containing proteins are commonplace in the apicomplexa and C. parvum possess 12 such proteins. Here, we explore the molecular evolution and conservation of these proteins and examine their abundance in C. parvum oocysts to assess the likelihood that they may be useful as vaccine candidates. We go onto examine the glycosylation states of these proteins using antibody-enabled and ZIC-HILIC enrichment techniques, which revealed that these proteins are modified with C-linked Hex and N-linked Hex5-6HexNAc2 glycans.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Cryptosporidium Parvum Iowa Ii

SUBMITTER: Nichollas Scott

LAB HEAD: Nichollas Scott

PROVIDER: PXD023935 | Pride | 2023-01-12

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	FAIMS_proteome_analysis.zip	Other
	Nsco_Crypto_proteome_FAIMS_20CV.raw	Raw
	Nsco_Crypto_proteome_FAIMS_30CV.raw	Raw
	Nsco_Crypto_proteome_FAIMS_40CV.raw	Raw
	Nsco_Crypto_proteome_FAIMS_50CV.raw	Raw

Items per page:

1 - 5 of 11

Similar Datasets

Exploring the glycosylation state of the Cryptosporidium parvum CpTSP protein family-HILIC glycopeptide enrichment

Project description:Cryptosporidium parvum is a zoonotic apicomplexan parasite and a common cause of diarrheal disease worldwide. The development of vaccines to prevent or limit infection remains an important goal for tackling these diarrheal diseases, which are a significant cause of infant morbidity in the developing world. The only approved vaccine against an apicomplexan parasite targets conserved adhesins possessing a thrombospondin repeat (TSR) domains. Orthologous TSR domain-containing proteins are commonplace in the apicomplexa and C. parvum possess 12 such proteins. Here, we explore the molecular evolution and conservation of these proteins and examine their abundance in C. parvum oocysts to assess the likelihood that they may be useful as vaccine candidates. We go onto examine the glycosylation states of these proteins using antibody-enabled and ZIC-HILIC enrichment techniques, which revealed that these proteins are modified with C-linked Hex and N-linked Hex5-6HexNAc2 glycans.

2023-01-12 | PXD023936 | Pride

Exploring the glycosylation state of the Cryptosporidium parvum CpTSP protein family-Cman pulldowns

2023-01-12 | PXD023934 | Pride

Comparison of OMV to membrane proteomes across different media (membrane experiments)

Project description:This project examines the impact of different growth media on the protein compositions of membranes of Bacteroides thetaiotaomicron.

2023-06-14 | PXD036272 | Pride

Comparsion of OMV to membrane proteomes across different media (OMV vs membrane experiments)

Project description:This project examines the impact of different growth media on the protein compositions of OMV and membrane of Bacteroides thetaiotaomicron.

2023-06-14 | PXD036181 | Pride

Identification of levoglucosan degradation pathways in bacteria

Project description:Levoglucosan is produced in the pyrolysis of cellulose and starch, including from bushfires or the burning of biofuels, and is deposited from the atmosphere across the surface of the earth. We describe two levoglucosan degrading Paenarthrobacter spp. (Paenarthrobacter nitrojuajacolis LG01 and Paenarthrobacter histidinolovorans LG02) that were isolated by metabolic enrichment on levoglucosan as sole carbon source. Genome sequencing and proteomics analysis revealed expression of a series of gene clusters encoding known levoglucosan degrading enzymes, levoglucosan dehydrogenase (LGDH, LgdA), 3-keto-levoglucosan b-eliminase (LgdB1) and glucose 3-dehydrogenase (LgdC), along with an ABC transporter cassette and associated solute binding protein. However, no homologues of 3-ketoglucose dehydratase (LgdB2) were evident. The expressed gene clusters contained a range of putative sugar phosphate isomerase/xylose isomerases with weak similarity to LgdB2. Sequence similarity network analysis of genome neighbors revealed that homologues of LgdA, LgdB1 and LgdC are generally conserved in a range of bacteria in the phyla Firmicutes, Actinobacteria and Proteobacteria. One sugar phosphate isomerase/xylose isomerase cluster (LgdB3) was identified with limited distribution mutually exclusive with LgdB2. LgdB1, LgdB2 and LgdB3 adopt similar predicted 3D folds suggesting overlapping function in processing intermediates in LG metabolism. Our findings highlight the diversity within the LGDH pathway through which bacteria utilize levoglucosan as a nutrient source.

2023-05-10 | PXD039086 | Pride

Proteomic impact of the loss of GH76 in TB

Project description:Proteomic impact of the loss of GH76 in TB by comparing WT to GH76

2024-06-04 | PXD042653 | Pride

A widespread family of oxidoreductive sulfoquinovosidases at the gateway to sulfoquinovose catabolism

Project description:Proteome analysis to study Sulfoquinovose catabolism Arthrobacter sp. strain AK01

2023-12-12 | PXD043482 | Pride

Bacteroides thetaiotaomicron OMV proteomics

Project description:Comparative proteomics of Bacteroides thetaiotaomicron samples comparing the total membrane (TM) and outer membrane vesicles (OMV) of WT B. thetaiotaomicron and delta 4364

2024-10-15 | PXD043219 | Pride

Comparison of OMV to membrane proteomes across different media (OMV)

Project description:This project examines the impact of different growth media on the protein compositions of OMV and membrane of Bacteroides thetaiotaomicron.

2023-06-14 | PXD036275 | Pride

Thrombospondin-1 type 1 repeats in a model of inflammatory bowel disease: genetic profile and therapeutic effects

Project description:Thrombospondin 1 (TSP-1) is an anti-angiogenic matricellular protein with regulatory functions in inflammation and cancer. The type 1 repeats (TSR) domains of TSP-1 have been shown to interact with a wide range of proteins that result in the anti-angiogenic and anti-tumor properties of TSP-1. To evaluate potential therapeutic effects of TSRs in inflammatory bowel disease, we conducted clinical, histological and gene microarray analyses on a mouse model of induced colitis. We used Affymetrix GeneChips to determine the changes in the genetic profile underlying TSR-treatment coincident with induction of colitis using DSS. We identified differentially expressed genes among the treatment groups. Using DSS (dextran sulfate sodium) to induce colitis, wild-type mice were simultaneously injected with either saline or one form of TSP-1 derived peptides, containing either the three domains of the TSR (3TSR), the two last domains (TSR2), or TSR2 with the RFK sequence (TSR2+RFK). Wt mice drinking water only was used as reference.

2014-08-26 | E-GEOD-32697 | biostudies-arrayexpress