Dataset Information

BoLA-DRimmunopeptidomics in Theileria parva infected, bovine cell lines

ABSTRACT: The ability to maintain T.parva-infected cell lines as rapidly proliferating cells in vitro that express high levels of both MHCI and MHCII, makes this a particularly amenable system in which to apply MHCp elution analysis. Furthermore, the ability of T. parva-infected cells to recall antigen-specific T-cells without the need for supplementary APCs indicates that the peptides presented by T. parva are those that stimulate T-cell responses. We have utilised the repertoire of bovine derived peptides from MHCp elution studies conducted on T. parva-infected cells to define the peptide binding motifs of a variety of BoLA-MHCI and BoLA-DR molecules and facilitated the development of novel more accurate peptide binding prediction algorithms.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive HF

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Cell Culture

DISEASE(S): Theileriasis

SUBMITTER: Nicola Ternette

LAB HEAD: Nicola Ternette

PROVIDER: PXD024053 | Pride | 2022-05-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2123_IL21_PEAKS_PTM_32_peptides_1_1_0.mzid.gz	Mzid
	2229_IL21_PEAKS_PTM_45_peptides_1_1_0.mzid.gz	Mzid
	2229_OVA_IL21_PEAKS_PTM_36_peptides_1_1_0.mzid.gz	Mzid
	2229_Tpm_IL21_PEAKS_PTM_40_peptides_1_1_0.mzid.gz	Mzid
	2824_IL21_PEAKS_PTM_43_peptides_1_1_0.mzid.gz	Mzid

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Publications

Integral Use of Immunopeptidomics and Immunoinformatics for the Characterization of Antigen Presentation and Rational Identification of BoLA-DR-Presented Peptides and Epitopes.

Fisch Andressa A Reynisson Birkir B Benedictus Lindert L Nicastri Annalisa A Vasoya Deepali D Morrison Ivan I Buus Søren S Ferreira Beatriz Rossetti BR Kinney Ferreira de Miranda Santos Isabel I Ternette Nicola N Connelley Tim T Nielsen Morten M

Journal of immunology (Baltimore, Md. : 1950) 20210331 10

MHC peptide binding and presentation is the most selective event defining the landscape of T cell epitopes. Consequently, understanding the diversity of MHC alleles in a given population and the parameters that define the set of ligands that can be bound and presented by each of these alleles (the immunopeptidome) has an enormous impact on our capacity to predict and manipulate the potential of protein Ags to elicit functional T cell responses. Liquid chromatography-mass spectrometry analysis of ...[more]

PMID: 33789985

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