Dataset Information

Proteomic response of Escherichia coli to membrane lytic and iron chelating Amaranthus tricolor defensin

ABSTRACT: Plant defensins are a broadly distributed family of antimicrobial peptides which have been primarily studied for agriculturally relevant antifungal activity. Recent studies have probed defensins against gram negative bacteria revealing evidence for multiple mechanisms of action including membrane lysis and ribosomal inhibition. In this study, a γ-core motif synthetic analog ( Atr-DEF2(G39-C55) ) of Amaranthus tricolor DEF2 (Atr-DEF2) is used to probe plant defensin antibacterial mechanism of action via proteomics.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Escherichia Coli

SUBMITTER: Leslie Hicks

LAB HEAD: Leslie M. Hicks

PROVIDER: PXD024138 | Pride | 2021-04-14

REPOSITORIES: Pride

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			Action	DRS
	20200608_TEB_2h_5-35_EC_atrdef1exp_C_1ug_1.raw	Raw
	20200608_TEB_2h_5-35_EC_atrdef1exp_C_1ug_3.raw	Raw
	20200608_TEB_2h_5-35_EC_atrdef1exp_C_1ug_4.raw	Raw
	20200608_TEB_2h_5-35_EC_atrdef1exp_C_1ug_5.raw	Raw
	20200608_TEB_2h_5-35_EC_atrdef1exp_H_1ug_2.raw	Raw

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Proteomic response of Escherichia coli to a membrane lytic and iron chelating truncated Amaranthus tricolor defensin.

Moyer Tessa B TB Purvis Ashleigh L AL Wommack Andrew J AJ Hicks Leslie M LM

BMC microbiology 20210412 1

<h4>Background</h4>Plant defensins are a broadly distributed family of antimicrobial peptides which have been primarily studied for agriculturally relevant antifungal activity. Recent studies have probed defensins against Gram-negative bacteria revealing evidence for multiple mechanisms of action including membrane lysis and ribosomal inhibition. Herein, a truncated synthetic analog containing the γ-core motif of Amaranthus tricolor DEF2 (Atr-DEF2) reveals Gram-negative antibacterial activity an ...[more]

PMID: 33845758

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