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BioID with P. falciparum protein PhiL1

ABSTRACT: The aim of the project was to identify proteins proximal to Plasmodium falciparum PhiL1

INSTRUMENT(S): Q Exactive

ORGANISM(S): Plasmodium Falciparum (isolate 3d7)

SUBMITTER: Frank Stein

LAB HEAD: Tim W. Gilberger

PROVIDER: PXD024237 | Pride | 2021-05-03

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	ACN_merged_results_20191021_0952_peptides.txt	Txt
	ACN_merged_results_20191021_0952_proteins.txt	Txt
	ACN_merged_results_20191021_0952_summary.txt	Txt
	Ambic_merged_results_20191021_1006_peptides.txt	Txt
	Ambic_merged_results_20191021_1006_proteins.txt	Txt

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1 - 5 of 31

Publications

Identification of novel inner membrane complex and apical annuli proteins of the malaria parasite Plasmodium falciparum.

Wichers Jan Stephan JS Wunderlich Juliane J Heincke Dorothee D Pazicky Samuel S Strauss Jan J Schmitt Marius M Kimmel Jessica J Wilcke Louisa L Scharf Sarah S von Thien Heidrun H Burda Paul-Christian PC Spielmann Tobias T Löw Christian C Filarsky Michael M Bachmann Anna A Gilberger Tim W TW

Cellular microbiology 20210516 9

The inner membrane complex (IMC) is a defining feature of apicomplexan parasites, which confers stability and shape to the cell, functions as a scaffolding compartment during the formation of daughter cells and plays an important role in motility and invasion during different life cycle stages of these single-celled organisms. To explore the IMC proteome of the malaria parasite Plasmodium falciparum we applied a proximity-dependent biotin identification (BioID)-based proteomics approach, using t ...[more]

PMID: 33830607

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Increased levels of the mitochondrial import factor Mia40 can prevent the aggregation of polyQ proteins in the cytosol

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Comparative_Interaction_Proteomics_GFP-tagged_MAGEC2_HEK293T

Project description:293T (Human embryonic kidney cells-ATCC CRL-3216) cells were plated at 500,000 cells per 10 cm dish and grown for 48 hours. Cells were then transfected with GFP-MAGEC1 WT or GFP-MAGEC1 L318D in a 3:1 ratio of PEI (polyethylenimine) to DNA. Cells were harvested after 48 hours and lysed in 50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100, protease inhibitor (cOmpleteâ„¢, Mini, EDTA-free (Roche)). Cells were incubated for 25 minutes on ice and then sonicated for 5 minutes at 4Â°C. Cells were centrifuged at 14,000 rpm for 20 minutes at 4 Â°C. 500 ug lysate was then added to GFP-TrapÂ® Agarose resin (ChromoTek) and samples were incubated rotating at 4Â°C for 2 hours. Following washing of the beads with 50 mM Tris pH 7.5, 150 mM NaCl, proteins were eluted by the addition of 2x Laemmli buffer and boiled for 10 minutes at 95 Â°C. Samples were spun down and the supernatants were sent for mass spectrometry analysis as described below. Experiments were conducted in triplicate.

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