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Profiling of sika deer antler proteins at different developmental stages based on label-free

ABSTRACT: We used label-free proteomics approach to analyze the protein expression dynamics of the antler tip in 6 developmental periods (15, 25, 45, 65, 100 and 130 days after the previous antler cast) and costal cartilage. the stages special proteins and differentially expressed proteins (DEPs) in different development stages were analyzed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Cervus Nippon Hortulorum

TISSUE(S): Cartilage, Antler

SUBMITTER: Ranran Zhang

LAB HEAD: Xiumei Xing

PROVIDER: PXD024323 | Pride | 2021-09-10

REPOSITORIES: Pride

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			Action	DRS
	P17406_B_100A1.raw	Raw
	P17406_B_100A2.raw	Raw
	P17406_B_100A3.raw	Raw
	P17406_B_130A1.raw	Raw
	P17406_B_130A2.raw	Raw

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Publications

Comparative antler proteome of sika deer from different developmental stages.

Zhang Ranran R Li Yang Y Xing Xiumei X

Scientific reports 20210518 1

Antler is a special bone tissue that has the ability to regenerate completely periodically. It is the fastest growing bone in the animal kingdom. Antler provides a valuable research model for bone growth and mineralization. Antler grows longitudinally by endochondral ossification with their growth center located in its tip. Many scholars have carried out detailed studies on morphology and gene expression of antler tip. However, few scholars have analyzed the protein expression patterns of antler ...[more]

PMID: 34006919

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comparative proteomics of deer antler

Project description:we used proteomic technology to disclose the difference of antler regeneration between red deer and sika deer. Through functional analysis, we obtained differentially expressed proteins and the pathway involved in antler regeneration between two groups

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Project description:Identification of the changes in gene expression between the antler velvet and mesenchyme, pedicle skin and frontal skin tissues from adult male deers (Cervus elaphus). Hybridization data were analysed using MAS5, RMA, GCRMA and Dchip algorithms to maximize the chances of identifying gene expression changes. Comparison of 4 tissues with 3 biological replicates each. Samples were obtained from Velvet, Mesenchyme, Pedicle, and Frontal tissues of 3 adult males.

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RNA-Seq of sika deer antler-tip during the ossification stage

Project description:To obtain an overview of the antler tip gene expression profile during rapid growth period, a cDNA sample was prepared from antler tip and sequenced using the Illumina sequencing platform.

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Transcription profiling of Cervus elaphus growing antler

Project description:The aim of the study is to characterize the gene expression of the growing antlers during their process of regeneration and fast growth Experiment Overall Design: Gene profiles of RNA samples from antler tip, base (pedicle) and frontal bone. Samples were harvested during spring, at the period of maximum antler growth (60 days after casting the previous antlers). And additional sample (DEER4) was harvested at the end of the growing season (90 days after the casting).

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Sequencing and de novo analysis of the Chinese Sika deer antler-tip transcriptome during the ossification stage using Illumina RNA-Seq Technology

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Project description:A small, shed antler fragment of a reindeer from Sjælland, Denmark has been dated to the Mid-Holocene, ca., 4700 cal B.C. Reindeer was an important component of the Lateglacial fauna in Denmark, and the species survived for ca. 1400 years into the Holocene. However, we consider it highly unlikely that this species inhabited Denmark during the Mid-Holocene, when dense forests characterized the vegetation and summer temperatures were somewhat higher than at present. We suggest that the reindeer antler came to Sjælland from Norway or Sweden as a result of trade, perhaps involving flint.

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