Proteomics

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Effect of starvation conditions on cultured human H4 neuroglioma cells stably expressing shLuc and shATG5

ABSTRACT: An attempt to analyse a large proportion of the human proteome of cultured Human H4 neuroglioma cells during conditions simulating starvation. Comparisons were made under conditions of ATG5 knock-down and control (Luc) knock-down. Whole proteome crude extracts were prepared and comparisons made using SILAC in both 'forward' and 'reverse' label arrangements.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Permanent Cell Line Cell

SUBMITTER: Mike Tatham  

LAB HEAD: Ronald T. Hay

PROVIDER: PXD024486 | Pride | 2022-04-04

REPOSITORIES: pride

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20160122_MT_ARF_Atg5_90m_01.raw Raw
20160122_MT_ARF_Atg5_90m_02.raw Raw
20160122_MT_ARF_Atg5_90m_03.raw Raw
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Publications

Negative Modulation of Macroautophagy by Stabilized HERPUD1 is Counteracted by an Increased ER-Lysosomal Network With Impact in Drug-Induced Stress Cell Survival.

Vargas Gabriela G   Cortés Omar O   Arias-Muñoz Eloisa E   Hernández Sergio S   Cerda-Troncoso Cristobal C   Hernández Laura L   González Alexis E AE   Tatham Michael H MH   Bustamante Hianara A HA   Retamal Claudio C   Cancino Jorge J   Varas-Godoy Manuel M   Hay Ronald T RT   Rojas-Fernández Alejandro A   Cavieres Viviana A VA   Burgos Patricia V PV  

Frontiers in cell and developmental biology 20220302

Macroautophagy and the ubiquitin proteasome system work as an interconnected network in the maintenance of cellular homeostasis. Indeed, efficient activation of macroautophagy upon nutritional deprivation is sustained by degradation of preexisting proteins by the proteasome. However, the specific substrates that are degraded by the proteasome in order to activate macroautophagy are currently unknown. By quantitative proteomic analysis we identified several proteins downregulated in response to s  ...[more]

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