Proteomics

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In-house packed porous graphitic carbon columns for liquid chromatography-mass spectrometry analysis of N-glycans

ABSTRACT: Although many reversed and normal stationary phases have been utilised for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner. Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 μm i.d., 360 μm o.d.) packed in-house with Hypercarb PGC (3 μm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS, Agilent instrument model

ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine)

SUBMITTER: Mark Condina  

LAB HEAD: Peter Hoffmann

PROVIDER: PXD024489 | Pride | 2021-07-08

REPOSITORIES: Pride

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20201125_N-gly-MQ-std-r005.mzdata Other
20201125_N-gly-MQ-std-r006.mzdata Other
20201125_N-gly-MQ-std-r007.mzdata Other
20201126_N-gly-MQ-std-r008.mzdata Other
20201126_N-gly-MQ-std-r009.mzdata Other
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In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of <i>N-</i>Glycans.

Young Clifford C   Condina Mark R MR   Briggs Matthew T MT   Moh Edward S X ESX   Kaur Gurjeet G   Oehler Martin K MK   Hoffmann Peter P  

Frontiers in chemistry 20210611

Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking. Altered glycosylation profiles are associated with cancer and inflammatory diseases, as well as impacting the efficacy of therapeutic monoclonal antibodies. Consisting of oligosaccharides attached to asparagine residues, enzymatically released <i>N-</i>linked glycans are analytically challenging due to the diversity of isomeric structures that  ...[more]

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