Proteomics

Dataset Information

0

Identification of specific proteins and metabolic networks associated with sucrose accumulation in sugarcane


ABSTRACT: The rate of improvement of sugar content in sugarcane remains low for decades worldwide. Our previous transcriptome studies provided an atlas of sucrose accumulation-related gene expression but little is known about proteins involved. Here this knowledge gap is addressed by a proteomic analysis of experimentally altered sucrose accumulation in sugarcane. Analysis of stem proteomes of ripener ethephon-treated high- and low-sugar genotypes had identified 2983 proteins of which 139 were significantly differentially expressed (DEPs). These DEPs were found to be involved in sugar metabolism-related processes with 25 of them may have a regulatory role in sucrose accumulation. The key proteins identified include UDP-glucose 6-dehydrogenase associated with amino sugar and nucleotide sugar metabolism; those involved in carbon fixation; and fructokinase, β-D-glucosidase and α-glucan phosphorylase involved in starch and sucrose metabolism. Distinct genotype- and ethephon-dependent DEP expression was evident providing new insights on one of the most intractable sugarcane traits to breeding.

INSTRUMENT(S): Q-Tof ultima

ORGANISM(S): Saccharum Spp. Complex Hybrid

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Dong-Liang Huang  

LAB HEAD: Dong-Liang Huang

PROVIDER: PXD024569 | Pride | 2022-08-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
29264-Subset_Database.fasta Fasta
GanZhe-1-1.raw Raw
GanZhe-1-2.raw Raw
GanZhe-1-3.raw Raw
GanZhe-10-1.raw Raw
Items per page:
1 - 5 of 39
altmetric image

Publications

Proteomics data analysis using multiple statistical approaches identified proteins and metabolic networks associated with sucrose accumulation in sugarcane.

Li Ao-Mei AM   Chen Zhong-Liang ZL   Qin Cui-Xian CX   Li Zi-Tong ZT   Liao Fen F   Wang Ming-Qiao MQ   Lakshmanan Prakash P   Li Yang-Rui YR   Wang Miao M   Pan You-Qiang YQ   Huang Dong-Liang DL  

BMC genomics 20220722 1


<h4>Background</h4>Sugarcane is the most important sugar crop, contributing > 80% of global sugar production. High sucrose content is a key target of sugarcane breeding, yet sucrose improvement in sugarcane remains extremely slow for decades. Molecular breeding has the potential to break through the genetic bottleneck of sucrose improvement. Dissecting the molecular mechanism(s) and identifying the key genetic elements controlling sucrose accumulation will accelerate sucrose improvement by molec  ...[more]

Similar Datasets

2021-12-30 | GSE188303 | GEO
2006-02-14 | GSE4233 | GEO
2016-12-31 | GSE44389 | GEO
2015-10-13 | GSE67897 | GEO
2021-09-09 | PXD027410 | Pride
2019-05-07 | GSE130757 | GEO
2008-07-01 | GSE11934 | GEO
2008-07-01 | E-GEOD-11934 | biostudies-arrayexpress
2010-06-30 | E-GEOD-14732 | biostudies-arrayexpress
2011-07-02 | E-GEOD-30296 | biostudies-arrayexpress