Dataset Information

Transcriptional landscape of Candida parapsilosis cells assimilating hydroxyaromatic substrates

ABSTRACT: The pathogenic yeast Candida parapsilosis can utilize a wide range of hydroxyaromatic substrates as sole carbon sources through the 3-oxoadipate pathway (3-OAP) and the gentisate pathway (GP). It was previously established that the genes coding for the components of these pathways are arranged in two metabolic gene clusters. In this study we investigated the transcriptional regulation of these gene clusters, as well as the changes in major metabolic pathways in cells assimilating hydroxyaromates. First, using second and third generation sequencing technologies we determined the genomic sequence of the C. parapsilosis CLIB214 strain. This was then used as a platform to a transcriptomic and proteomic analysis of CLIB214 cells utilizing different substrates of the 3-OAP and the GP. Our results show that the activation of both pathways leads to upregulation of genes related to β-oxidation of fatty acids, glyoxylate cycle, metabolism of amino acids and the biogenesis of peroxisomes. In spite of the similar overall response, we identified quite the difference in gene expression between cells assimilating individual aromatic substrates and surprisingly, not only when comparing the substrates of the 3OAP with the GP substrates, but also between substrates channelled through the same pathway. Moreover, with the phenotype analysis of knockout mutants, we showed evidence that transcription factors Otf1 and Gtf1 function as transcriptional activators of genes coding for core components of the 3-OAP and GP, respectively.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Candida Parapsilosis

SUBMITTER: Peter Barath

LAB HEAD: Peter Barath

PROVIDER: PXD024608 | Pride | 2022-02-01

REPOSITORIES: Pride

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Transcriptome and proteome profiling reveals complex adaptations of Candida parapsilosis cells assimilating hydroxyaromatic carbon sources.

Cillingová Andrea A Tóth Renáta R Mojáková Anna A Zeman Igor I Vrzoňová Romana R Siváková Barbara B Baráth Peter P Neboháčová Martina M Klepcová Zuzana Z Brázdovič Filip F Lichancová Hana H Hodorová Viktória V Brejová Broňa B Vinař Tomáš T Mutalová Sofia S Vozáriková Veronika V Mutti Giacomo G Tomáška Ľubomír Ľ Gácser Atilla A Gabaldón Toni T Nosek Jozef J

PLoS genetics 20220307 3

Many fungal species utilize hydroxyderivatives of benzene and benzoic acid as carbon sources. The yeast Candida parapsilosis metabolizes these compounds via the 3-oxoadipate and gentisate pathways, whose components are encoded by two metabolic gene clusters. In this study, we determine the chromosome level assembly of the C. parapsilosis strain CLIB214 and use it for transcriptomic and proteomic investigation of cells cultivated on hydroxyaromatic substrates. We demonstrate that the genes coding ...[more]

PMID: 35255079

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