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Species identification of silks by protein mass spectrometry

ABSTRACT: We investigate a range of methods for solubilizing modern and archaeological silks and demonstrate a protocol using mass spectrometry-based proteomics to successfully differentiate modern samples of Bombyx, Antheraea, and Samia -produced silks matching samples to species level. We also analyzed archaeological silks from excavations at the ancient city of Palmyra, Syria, and provide evidence that these silks were produced by A. mylitta, which is the first direct evidence supporting the production and trade of Indian wild silk in antiquity.

INSTRUMENT(S): Waters instrument model

ORGANISM(S): Bombyx Mori (silk Moth) Antheraea Mylitta Samia Ricini Antheraea Pernyi Antheraea Yamamai Antheraea Assamensis

SUBMITTER: Boyoung Lee

LAB HEAD: Boyoung Lee

PROVIDER: PXD024610 | Pride | 2022-04-04

REPOSITORIES: Pride

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			Action	DRS
	1-1.mgf	Mgf
	1B.mgf	Mgf
	3-2K.mgf	Mgf
	3B.mgf	Mgf
	4-1.mgf	Mgf

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Publications

Species identification of silks by protein mass spectrometry reveals evidence of wild silk use in antiquity.

Lee Boyoung B Pires Elisabete E Pollard A Mark AM McCullagh James S O JSO

Scientific reports 20220317 1

Silk has been a luxurious commodity throughout modern human history and sericulture has played an important role in ancient global trade as well as technological and cultural developments. Archaeological findings suggest that prior to domestication of the mulberry silkworm (Bombyx mori) silks were obtained from a range of silk-producing moth species with regional specificity. However, investigating the origins of sericulture is difficult as classification of silks by species-type has proved tech ...[more]

PMID: 35301348

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Species identification of silks by protein mass spectrometry

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O-GlcNAcylation regulates epidermal growth factor receptor intracellular trafficking and signaling

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Lipoylation is dependent on the ferredoxin FDX1 and dispensable under hypoxia in human cells

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