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Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair

ABSTRACT: Now a days, wound healing is becoming a global threat that impact economy of the country severely. Though the steps involved in wound healing are well characterised, direct therapeutics for the accelerated wound healing is comparatively less. This is solely because of the incomplete mechanism of healing and the lack of knowledge on molecular players involved in wound healing. Hence, in the present study, we have investigated the molecular players involved in wound healing process using a versatile model organism Caenorhabditis elegans through proteomic analyses. Especially, we have employed the high through put proteomic analyses tools such as 2-D GE and LCMS/MS analysis to uncover the molecular players involved in wound healing. As a result of 2-D GE analysis, a total of 35 differentially regulated proteins were identified during injury in which 22 and 13 proteins were upregulated and downregulated, respectively. Bioinformatics analyses result suggested that the upregulated proteins have crucial roles in serotonin/ acetyl choline synthesis and calcium signalling, whereas downregulated proteins were majorly involving in ubiquitin mediated proteolysis. Additionally, the mRNA levels of up and downregulated proteins were validated using qPCR analysis. Overall, the study suggested that injury initiates multiple molecular mechanisms to repair the damage.

INSTRUMENT(S): AXIMA Performance MALDI-TOF/TOF

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

DISEASE(S): Wounds And Injuries

SUBMITTER: Krishnaswamy Balamurugan

LAB HEAD: Dr. KB Lab (Host Pathogen Interaction lab)

PROVIDER: PXD024629 | Pride | 2021-04-16

REPOSITORIES: Pride

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Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair

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