Proteomics

Dataset Information

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Rat myocardial left ventricular tissue proteins LC-MSMS


ABSTRACT: Rat left ventricular tissue LC-MSMS.Rat left ventricular tissue proteins from different groups were extracted and quantified using the BCA Protein Assay Kit (Thermo Fisher Scientific, USA). The proteins were then labeled with tandem mass tags (TMT). TMT6/10 (Pierce, USA) with different reporter ions (126-131 Da) were applied as isobaric tags for relative quantification. Then the labeled peptide aliquots were combined for fractionation and further LC-MS analysis. The LC–MS/MS analysis was carried out in Capitbalbio Technology by using Q Exactive mass spectrometer (Thermo Fisher Scientific, USA). Twenty most intense precursor ions from a survey scan were selected for MS/MS detected in Orbitrap analyser. All the tandem mass spectra were produced by higher-energy collision dissociation (HCD) method. The MS/MS data was collected and searched against the Oryctolagus cuniculus database using the Sequest algorithms. A protein with fold change≥1.5, and the presence of least 2 unique peptides with a P value <0.05, was considered to be differentially expressed protein (DEP). Finally, DEPs were analyzed by reference to three key databases: Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome and the Clusters of Orthologous Groups (COG).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart

SUBMITTER: Xiaohong Wei  

LAB HEAD: Jingyan Han

PROVIDER: PXD024641 | Pride | 2021-07-26

REPOSITORIES: Pride

Dataset's files

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Publications

Quantitative Proteomics Reveal That Metabolic Improvement Contributes to the Cardioprotective Effect of T<sub>89</sub> on Isoproterenol-Induced Cardiac Injury.

Wei Xiao-Hong XH   Guo Xiao X   Pan Chun-Shui CS   Li Huan H   Cui Yuan-Chen YC   Yan Li L   Fan Jing-Yu JY   Deng Jing-Na JN   Hu Bai-He BH   Chang Xin X   He Shu-Ya SY   Yan Lu-Lu LL   Sun Kai K   Wang Chuan-She CS   Han Jing-Yan JY  

Frontiers in physiology 20210628


<h4>Background</h4>T<sub>89</sub>, a traditional Chinese medicine, has passed phase II, and is undergoing phase III clinical trials for treatment of ischemic cardiovascular disease by the US FDA. However, the role of T<sub>89</sub> on isoproterenol (ISO)-induced cardiac injury is unknown. The present study aimed to explore the effect and underlying mechanism of T<sub>89</sub> on ISO-induced cardiac injury.<h4>Methods</h4>Male Sprague-Dawley rats received subcutaneous injection of ISO saline solu  ...[more]

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