Dataset Information

TDFragMapper: Evaluating experimental parameters for confident intact protein characterization in top-down proteomics

ABSTRACT: New software-tool allowing an easy visualization of fragment ions and thus a rapid evaluation of key experimental parameters on the sequence coverage obtained for the MS/MS analysis of intact proteins. Our tool can deal with multiple fragmentation methods. We demonstrate that TDFragMapper can rapidly highlight the experimental fragmentation parameters that are critical to the characterization of intact proteins of various size using top-down proteomics.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine) Escherichia Coli Streptococcus Dysgalactiae

SUBMITTER: Jonathan Dhenin

LAB HEAD: Julia Chamot-Rooke

PROVIDER: PXD024643 | Pride | 2021-12-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20200730_L_JD_ProtMix_tMS2_ET10hcD10_IGF1-ET15hcD15_High_01.raw	Raw
	20200730_L_JD_ProtMix_tMS2_ET10hcD10_IGF1-ET15hcD15_Low_01.raw	Raw
	20200730_L_JD_ProtMix_tMS2_ET10hcD10_IGF1_ET15hcD15_High_01.zip	Other
	20200730_L_JD_ProtMix_tMS2_ET10hcD10_IGF1_ET15hcD15_Low_01.zip	Other
	20200730_L_JD_ProtMix_tMS2_ET15hcD15_IGF1-ET20hcD15_High_01.raw	Raw

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Publications

TDFragMapper: a visualization tool for evaluating experimental parameters in top-down proteomics.

Dhenin Jonathan J Borges Lima Diogo D Dupré Mathieu M Chamot-Rooke Julia J

Bioinformatics (Oxford, England) 20220101 4

<h4>Motivation</h4>We present a new software-tool allowing an easy visualization of fragment ions and thus a rapid evaluation of key experimental parameters on the sequence coverage obtained for the MS/MS (tandem mass spectrometry) analysis of intact proteins. Our tool can process data obtained from various deconvolution and fragment assignment software.<h4>Results</h4>We demonstrate that TDFragMapper can rapidly highlight the experimental fragmentation parameters that are critical to the charac ...[more]

PMID: 34792554

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