Proteomics

Dataset Information

0

Proteomic Analysis of Functional Inward Rectifier Potassium Channel (Kir) 2.1 for Posttranslational Modification Discovery


ABSTRACT: Kir2.1 has been implicated in a number of channelopathies including Andersen-Tawil Syndrome, Short QT Syndrome and catecholaminergic polymorphic ventricular tachycardia. We developed a mass spectrometry-based assay to identify phosphorylation sites and test the functional consequence of removing those sites by site-directed mutagenesis. Our study identified novel sites of phosphorylation and suggests that the site of phosphorylation can influence channel function. We envision our approach can be readily adapted to study additional mutations and other ion channels.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: kyle brown  

LAB HEAD: Ying Ge

PROVIDER: PXD024662 | Pride | 2021-11-03

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Kir21S425A_TDP_PKA_1.baf Other
Kir21S425A_TDP_PKA_2_ETD.baf Other
Kir21S425A_trypsin_1.tdf Other
Kir21S425A_trypsin_2.tdf Other
Kir21S425A_trypsin_3.tdf Other
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