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Shotgun proteomic analysis of the marine cyanobacterium Prochlorococcus sp. MED4 exposed to titanium dioxide nanoparticles

ABSTRACT: Cell- and extracellular proteomes obtained from Prochlorococcus sp. MED4 cultures exposed to nTiO2 were analysed to investigate any molecular features of toxicity.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Prochlorococcus Sp.

TISSUE(S): Cell Culture

SUBMITTER: Craig Dedman

LAB HEAD: Joseph A. Christie-Oleza

PROVIDER: PXD024726 | Pride | 2021-09-10

REPOSITORIES: Pride

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			Action	DRS
	OT_191122_37_MS19-242_Cell_C1.raw	Raw
	OT_191122_37_MS19-242_Cell_C2.raw	Raw
	OT_191122_37_MS19-242_Cell_C3.raw	Raw
	OT_191122_37_MS19-242_Cell_T1.raw	Raw
	OT_191122_37_MS19-242_Cell_T2.raw	Raw

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Publications

Environmentally relevant concentrations of titanium dioxide nanoparticles pose negligible risk to marine microbes.

Dedman Craig J CJ King Aaron M AM Christie-Oleza Joseph A JA Davies Gemma-Louise GL

Environmental science. Nano 20210409 5

Nano-sized titanium dioxide (nTiO<sub>2</sub>) represents the highest produced nanomaterial by mass worldwide and, due to its prevalent industrial and commercial use, it inevitably reaches the natural environment. Previous work has revealed a negative impact of nTiO<sub>2</sub> upon marine phytoplankton growth, however, studies are typically carried out at concentrations far exceeding those measured and predicted to occur in the environment currently. Here, a series of experiments were carried o ...[more]

PMID: 34046180

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The transcriptome landscape of Prochlorococcus MED4 and the factors for stabilizing the core genome

Project description:RNA-Seq technique was used to obtain the transcriptome map of Prochlorococcus MED4, including operons, untranslated regions, non-coding RNAs, and novel genes. Genome-wide expression profiles revealed that three factors contribute to core genome stabilization. First, a negative correlation between gene expression levels and protein evolutionary rates was observed. Highly expressed genes were overrepresented in the core genome but not in the flexible genome. Gene necessity was determined as a second powerful constraint on genome evolution through functional enrichment analysis. Third, quick mRNA turnover may increase corresponding proteinsM-bM-^@M-^Y fidelity among genes that were abundantly expressed. Together, these factors influence core genome stabilization during MED4 genome evolution. The cells were cultured on Pro99 medium for a certain time course (1, 3, 4, 8, and 10 days); or transferred to AMP (Artificial Medium for Prochlorococcus) with 6 mM or 24 mM sodium bicarbonate for 5 or 10 hours.

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