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Radiation resistance comparable to natural extremophiles achieved through experimental evolution

ABSTRACT: Recent human exploitation of high-level sources of ionizing radiation (IR) has generated a corresponding need to understand the effects of IR on living systems. Part of that research has focused on the astonishing capacity of some organisms to survive extraordinary levels of IR exposure. Using experimental evolution, we have generated populations of Escherichia coli with an IR resistance phenotype comparable to the extremophile Deinococcus radiodurans. Every aspect of cell physiology is affected. Cellular isolates exhibit approximately 1,000 base pair changes plus major genomic and proteomic alterations. The extreme IR resistance phenotype, stable without selection for at least 100 generations, has no single molecular origin. Defined and probable contributions include alterations in cellular systems involved in DNA repair, amelioration of reactive oxygen species, Fe metabolism and repair of iron-sulfur centers, DNA packaging, intermediary metabolism, and more. Some contributions to IR resistance are highly dependent on both genomic and environmental context.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Deinococcus Radiodurans Escherichia Coli

SUBMITTER: Benjamin Minkoff

LAB HEAD: Michael R Sussman

PROVIDER: PXD024784 | Pride | 2021-09-10

REPOSITORIES: pride

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Proteome Damage Inflicted by Ionizing Radiation: Advancing a Theme in the Research of Miroslav Radman.

Bruckbauer Steven T ST Minkoff Benjamin B BB Sussman Michael R MR Cox Michael M MM

Cells 20210420 4

Oxidative proteome damage has been implicated as a major contributor to cell death and aging. Protein damage and aging has been a particular theme of the recent research of Miroslav Radman. However, the study of how cellular proteins are damaged by oxidative processes is still in its infancy. Here we examine oxidative changes in the proteomes of four bacterial populations-wild type <i>E. coli</i>, two isolates from <i>E. coli</i> populations evolved for high levels of ionizing radiation (IR) res ...[more]

PMID: 33924085

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