Dataset Information

Histone post-translational modification analysis from low-abundance clinical samples

ABSTRACT: We streamlined the protein extraction procedure from low-amount clinical samples and tested and implemented different in-gel digestion strategies, obtaining a protocol that allows the analysis of the most common histone PTMs from laser microdissected tissue areas containing as low as 1000 cells. We then applied this protocol to breast cancer patient tissue sections in two proof-of-concept experiments, identifying differences in histone marks in heterogeneous regions selected by either morphological evaluation or MALDI-imaging profiling. These results demonstrate that analyzing histone PTMs from very small tissue areas and detecting differences from adjacent tumor regions is technically feasible, opening the way for the investigation of epigenetic features in the context of tissue and tumor heterogeneity.

INSTRUMENT(S): Q Exactive Plus, Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Breast Epithelium, Acinar Cell, Breast, Pancreas, Breast Cancer Cell

DISEASE(S): Breast Cancer

SUBMITTER: Roberta Noberini

LAB HEAD: Tiziana Bonaldi

PROVIDER: PXD024799 | Pride | 2021-09-09

REPOSITORIES: Pride

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Spatial epi-proteomics enabled by histone post-translational modification analysis from low-abundance clinical samples.

Noberini Roberta R Savoia Evelyn Oliva EO Brandini Stefania S Greco Francesco F Marra Francesca F Bertalot Giovanni G Pruneri Giancarlo G McDonnell Liam A LA Bonaldi Tiziana T

Clinical epigenetics 20210728 1

<h4>Background</h4>Increasing evidence linking epigenetic mechanisms and different diseases, including cancer, has prompted in the last 15 years the investigation of histone post-translational modifications (PTMs) in clinical samples. Methods allowing the isolation of histones from patient samples followed by the accurate and comprehensive quantification of their PTMs by mass spectrometry (MS) have been developed. However, the applicability of these methods is limited by the requirement for subs ...[more]

PMID: 34315505

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