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The proteome and phosphoproteome of acute stress in the mouse hippocampus

ABSTRACT: The acute stress response mobilizes energy to meet situational demands and re-establish homeostasis. However, little is known about the nature and dynamics of the underlying molecular cascades. We used a brief forced swim exposure to trigger a strong stress response in mice, which transiently increases anxiety, but does not lead to lasting maladaptive behavioral changes. Using multiomic profiling we characterize the stress-induced molecular events in the hippocampus over time, across molecular scales, and down to the level of cell types and single cells. Our results unveil the complexity and specificity of the healthy stress response, with widespread changes in protein phosphorylation and gene transcription, but tightly regulated protein translation. All observed molecular events resolve efficiently within 4 hours after initiation of stress.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hippocampal Formation, Hippocampus

DISEASE(S): Acute Stress Disorder

SUBMITTER: Lukas von Ziegler

LAB HEAD: Johannes Bohacek

PROVIDER: PXD024829 | Pride | 2022-02-09

REPOSITORIES: Pride

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			Action	DRS
	20180504_03_S178290_20180502_4HFST_11dCA1_re.raw	Raw
	20180504_04_S178291_20180502_4HFST_11vCA1_re.raw	Raw
	20180504_07_S178292_20180502_4HFST_11vCA3.raw	Raw
	20180504_08_S178293_20180502_4HFST_12vDG.raw	Raw
	20180504_10_S178294_20180502_4HFST_12dCA1.raw	Raw

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The proteome and phosphoproteome of acute stress in the mouse hippocampus

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