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Bacillus cereus msrABmsrA double mutant strain proteomics under fermentative anoxic conditions, cellular proteome

ABSTRACT: This project provides tandem mass spectrometry datasets of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid devoided of both the methionine sulfoxide reductase AB (msrAB)and the methionine sulfoxide reductase A (msrA) genes. The double mutant strain was grown under fermentative anaerobic condition and harvested at three growth stages.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Bacillus Cereus (strain Atcc 14579 / Dsm 31)

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD024849 | Pride | 2021-09-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	BCRUS09_20130527.fasta	Fasta
	F212330.dat	Other
	F212330_p0.05.mzid.gz	Mzid
	F212331.dat	Other
	F212331_p0.05.mzid.gz	Mzid

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1 - 5 of 38

Publications

Methionine Sulfoxide Reductases Contribute to Anaerobic Fermentative Metabolism in <i>Bacillus cereus</i>.

Duport Catherine C Madeira Jean-Paul JP Farjad Mahsa M Alpha-Bazin Béatrice B Armengaud Jean J

Antioxidants (Basel, Switzerland) 20210520 5

Reversible oxidation of methionine to methionine sulfoxide (Met(O)) is a common posttranslational modification occurring on proteins in all organisms under oxic conditions. Protein-bound Met(O) is reduced by methionine sulfoxide reductases, which thus play a significant antioxidant role. The facultative anaerobe <i>Bacillus cereus</i> produces two methionine sulfoxide reductases: MsrA and MsrAB. MsrAB has been shown to play a crucial physiological role under oxic conditions, but little is known ...[more]

PMID: 34065610

Similar Datasets

Bacillus cereus msrABmsrA double mutant strain proteomics under fermentative anoxic conditions, exoproteome

Project description:This project provides tandem mass spectrometry datasets of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid devoided of both the methionine sulfoxide reductase AB (msrAB)and methionine sulfoxide reductase A (msrA) genes. The double mutant strain was grown under fermentative anaerobic condition and harvested at three growth stages.

2021-09-10 | PXD024848 | Pride

Bacillus cereus msrAB mutant strain proteomics under fermentative anoxic conditions, exoproteome

Project description:This project provides tandem mass spectrometry datasets of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid and devoided of the methionine sulfoxide reductase AB (msrAB) gene. The single mutant strain was grown under fermentative anaerobic condition and harvested at three growth stages.

2021-09-10 | PXD024714 | Pride

Bacillus cereus msrAB mutant strain proteomics under fermentative anoxic conditions, cellular proteome

Project description:his project provides tandem mass spectrometry datasets of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid devoided of the methionine sulfoxide reductase AB (msrAB) gene. The single mutant strain was grown under fermentative anaerobic condition and harvested at three growth stages.

2021-09-10 | PXD024927 | Pride

Bacillus cereus msrA mutant strain proteomics under fermentative anoxic conditions, exoproteome

Project description:This project provides tandem mass spectrometry datasets of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid and devoided of the methionine sulfoxide reductase A (msrA) gene. The single mutant strain was grown under fermentative anaerobic condition and harvested at three growth stages.

2021-09-10 | PXD024847 | Pride

Bacillus cereus msrA mutant strain proteomics under fermentative anoxic conditions, cellular proteome

Project description:This project provides tandem mass spectrometry datasets of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid devoided of the methionine sulfoxide reductase A (msrA) gene. The single mutant strain was grown under fermentative anaerobic condition and harvested at three growth stages.

2021-09-10 | PXD024850 | Pride

Bacillus cereus proteomics, extraproteome

Project description:This project contributes to the proteomic comparison of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid, its mutant strain devoided of the methionine sulfoxide reductase AB (msrAB) protein and the corresponding msrAB complemented strain, all strains grown in aerobiosis condition and harvested at three growth stages.

2018-06-15 | PXD006169 | Pride

Bacillus cereus proteomics, intraproteome

2018-06-15 | PXD006205 | Pride

Mycobacterium smegmatis secreting methionine sulfoxide reductase A (MsrA) modulates cellular processes in mouse macrophages

Project description:Methionine sulfoxide reductase A (MsrA) is an antioxidant repair enzyme that reduces the oxidized methionine (Met-O) in proteins to methionine (Met). It plays a pivotal role in the cellular processes has been well established by overexpressing, silencing and knocking down of MsrA or by deleting the gene encoding MsrA in several species. We are specifically interested in understanding the role of secreted MsrA of bacterial pathogens. To elucidate this, we infected mouse bone marrow derived macrophages (BMDMs) with recombinant Mycobacterium smegmatis strain (MSM) secreting a bacterial MsrA or M. smegmatis strain (MSC) carrying only the control vector. BMDMs infected with MSM induced higher levels of ROS and TNF-α than BMDMs infected with MSC. The increased ROS and TNF-α in BMDMs infected with MSM correlated with elevated necrotic cell death. Further, RNA-Seq transcriptome analysis of BMDMs infected with MSC and MSM revealed differential expression of protein and RNA coding genes, suggesting that bacterial delivered MsrA could modulate the host cellular processes. Finally, KEGG pathway enrichment analysis identified down-regulation of cancer related signaling genes in MSM infected cells, indicating that MsrA has the potential to regulate the development and progression of cancer.

2023-08-28 | GSE212228 | GEO

Next Generation Sequencing of Wild Type and Mice Knocked Out for Known Methionine Sulfoxide Reducatases

Project description:Methionine sulfoxide reductase (Msr) is an important antioxidant enzyme. The mammalian Msr family has four members: MsrA, MsrB1, MsrB2, and MsrB3. We generated a mouse strain with deletion of all four Msr proteins. Deletion was confirmed by Western blotting. The quadruple knockout mouse is viable, and growth, development, and fertility appear normal. However, they are unexpectedly resistanct to oxidative stresses. We therefore determined the RNA expression profile in liver and heart of wild-type and quadruple knockout mice.

2019-10-01 | GSE130394 | GEO

The fused methionine sulfoxide reductase MsrAB promotes oxidative stress defense and bacterial virulence in Fusobacterium nucleatum

Project description:Recognizably associated with oral infections, adverse pregnancy outcomes, and colorectal cancer, the Gram-negative oral bacterium Fusobacterium nucleatum is known to colonize many extraoral sites including placenta and colon. How this anaerobe copes with oxidative stress imposed by the host immune response in various tissues is unknown. Here, we revealed that F. nucleatum harbors a gene locus coding for a single, fused methionine sulfoxide reductase (MsrAB), a two-component transduction system (ModRS), thioredoxin (Trx)- and cytochrome c (CcdA)-like proteins. Considering their role in oxidative stress responses, we demonstrated that these genes were increasingly expressed when fusobacterial cells were exposed to hydrogen peroxide. Comparative transcriptome analysis of the parental and modR mutant strains revealed that the response regulator ModR regulates a large regulon including trx, ccdA, and many metabolic genes. Significantly, mutants lacking individual genes within the msrAB locus, including msrAB, were sensitive to reactive oxygen species and defective in adherence/invasion of colorectal epithelial cells, as compared to the parental strain. The msrAB mutant was also defective in survival in macrophage, unlike the rescued and parental strains. Strikingly, in an experimental model of preterm birth this mutant was severely attenuated in virulence, for it failed to colonize the placenta and spread to the amniotic fluid. The results support that the MsrAB system represents a major pathway that protects fusobacteria against oxidative damage and confers virulence and that MsrAB-mediated oxidative stress defense is subjected to regulation by the two-component transduction system ModRS.

2022-03-28 | GSE174320 | GEO

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