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Helicobacter pylori outer membrane vesicle proteome

ABSTRACT: Gram negative bacteria release outer membrane vesicles (OMVs) as part of their natural growth. These OMVs take part in a biological functions including bacterial communication, exchange of genetic material and bacterial pathogenesis. However, the relationship between bacterial growth stage and OMV protein composition has not been explored before nor how their proteome is different to their parent bacterium. In this study, we examined the proteome of Helicobacter pylori and its OMVs from early log, late log or stationary phase of growth and found that globally, the protein content of OMVs over time is vastly different to one another as well as to their parent bacterium. OMVs purified from early log phase of growth contained the greatest number of unique proteins and a significant upregulation of virulence and pathogenic proteins. However, when compared back to their parent bacterium, OMVs from later stages of growth contained more unique proteins than those from earlier stages of growth. We show for the first time the regulation of OMV protein composition by H. pylori that is dependent on bacterial growth stage. Our results have expanded our understanding of the fundamental production of OMVs and the selective packaging of cargo in OMVs that result in specific functions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Helicobacter Pylori J99 (campylobacter Pylori J99)

TISSUE(S): Cell Culture

DISEASE(S): Disease Free

SUBMITTER: david greening

LAB HEAD: David Greening

PROVIDER: PXD024918 | Pride | 2021-03-23

REPOSITORIES: Pride

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			Action	DRS
	DG_1A_20180609151354.raw	Raw
	DG_1B.raw	Raw
	DG_1C.raw	Raw
	DG_2A.raw	Raw
	DG_2B.raw	Raw

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Project description:OMVs were isolated from H. pylori grown during pH 5.3 conditions with the addition of urea to supplement bacterial growth, or during pH 7.2 conditions with or without the addition of urea. Growth of H. pylori during acidic conditions reduced the quantity and size of OMVs produced. Additionally, acidic growth conditions resulted in the production of OMVs with increased packaging of protein, DNA, and RNA compared to OMVs produced during neutral growth conditions. A global data-independent acquisition proteomic analysis comparing the proteomes of OMVs to their parent bacteria demonstrated significant differences in the enrichment of proteins within bacteria and OMVs, supporting that differing conditions of growth impacts OMV composition. We also identified select and marginal differences in the enrichment of proteins within OMVs produced during different pH growth conditions. Overall, our findings reveal that growth of H. pylori during altered pH growth conditions is a factor that determines OMV proteomes, which may affect their subsequent functions in the host.

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