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Proteomic analysis of exoproteomes of human cells upon bystander effects induced by irradiated chondrosarcoma cells

ABSTRACT: Next-generation proteomics of proteins from the medium of human cells in culture after incubation with the medium from irradiated chondrosarcoma cells.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Chondrocyte, Cell Culture

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean ARMENGAUD

PROVIDER: PXD024953 | Pride | 2021-09-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	F211002.dat	Other
	F211002_p0.05.mzid.gz	Mzid
	F211004.dat	Other
	F211004_p0.05.mzid.gz	Mzid
	F211006.dat	Other

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Publications

A Proteomic Study Suggests Stress Granules as New Potential Actors in Radiation-Induced Bystander Effects.

Tudor Mihaela M Gilbert Antoine A Lepleux Charlotte C Temelie Mihaela M Hem Sonia S Armengaud Jean J Brotin Emilie E Haghdoost Siamak S Savu Diana D Chevalier François F

International journal of molecular sciences 20210726 15

Besides the direct effects of radiations, indirect effects are observed within the surrounding non-irradiated area; irradiated cells relay stress signals in this close proximity, inducing the so-called radiation-induced bystander effect. These signals received by neighboring unirradiated cells induce specific responses similar with those of direct irradiated cells. To understand the cellular response of bystander cells, we performed a 2D gel-based proteomic study of the chondrocytes receiving th ...[more]

PMID: 34360718

Similar Datasets

Proteomic analysis of bystander effects induced by irradiated chondrosarcoma cells

Project description:To understand the cellular response of bystander cells, we performed a 2D gel-based proteomic study of the chondrocyte receiving the conditioned medium of low dose irradiated chondrosarcoma cells. The proteomic analysis of the bystander chondrocytes highlighted 20 proteins spots that were significantly modified at low dose, implicating several cellular mechanisms, such as oxidative stress responses, cellular motility and exosomes pathways. Several of these candidates and cellular mechanisms were confirmed by functional analysis, such as 8-oxodG quantification, western blot and wound-healing migration tests.

2021-09-10 | PXD025187 | Pride

Bacillus cereus AH187 proteomics grown in glucose containing medium, exoproteome

Project description:This project provides tandem mass spectrometry data of Bacillus cereus AH187 strain. The strain was grown on liquid MOD medium supplemented with glucose and harvested at three growth stages.

2022-02-22 | PXD030165 | Pride

Bacillus cereus strain proteomics grown in glucose containing medium, cellular proteome

2022-02-22 | PXD030118 | Pride

Bacillus cereus ctaA mutant strain proteomics grown in glucose containing medium, cellular proteome

Project description:This project provides tandem mass spectrometry data of Bacillus cereus AH187 strain devoid of the heme A synthase (CtaA) protein. The mutant strain was grown on liquid MOD medium supplemented with glucose and harvested at three growth stages.

2022-02-22 | PXD030114 | Pride

Bacillus cereus ctaA mutant strain proteomics grown in glucose containing medium, exoproteome

2022-02-22 | PXD030163 | Pride

bystander effects from irradiated mouse brain

Project description:control: sham irradiated test1: mouse whole brain irradiated 10Gy for once test2:mouse whole brain irradiated 10Gy for 3 times, once a week

2021-01-24 | MSV000086761 | MassIVE

Surface proteome of emetic Bacillus cereus strain grown in glucose containing medium

Project description:This project provides tandem mass spectrometry data of extracellular non-covalently bound cell surface proteins from samples of Bacillus cereus AH187 strain. The wild-type strain was grown on liquid MOD medium supplemented with glucose and harvested at five growth stages: early exponential, late exponential, stationary phase, tardive stationary 1 and tardive stationary 2.

2022-08-12 | PXD033486 | Pride

Surface proteome of emetic Bacillus cereus delta-sl2 delta-eag double mutant strain grown in glucose containing medium

Project description:his project provides tandem mass spectrometry data of extracellular non-covalently bound cell surface proteins from samples of Bacillus cereus AH187 devoid of the S-layer 2 (SL2) and extractable antigen 1 (EA1) S-layer proteins. The double mutant strain was grown on liquid MOD medium supplemented with glucose and harvested at five growth stages: early exponential, late exponential, stationary phase, tardive stationary 1 and tardive stationary 2.

2022-08-12 | PXD033494 | Pride

Frankia coriariae exoproteomes

Project description:This project defines the exoproteome dynamics of Frankia coriariae, a nitrogen-fixing bacterium, in presence of three host plants. Frankia coriariae was treated with root exudates from compatible (Coriaria myrtifolia), incompatible (Alnus glutinosa) and non-actinorhizal (Cucumis melo) host plants.

2017-05-19 | PXD005980 | Pride

Transcription profiling by array of irradated and non-irradiated human K562 human erythroleukemic cells to study the effect of ionizing radiation and the bystander effect

Project description:The objective of the experiment was to compare changes in the transcriptome induced by direct X-irradiation of cells and by factors released by irradiated cells into the culture medium (irradiation conditioned medium, ICM), using the K562 human erythroleukemic cell line. Two culture flasks with K562 cells (5 x 105 cells/ml) were irradiated with X-rays at the dose of 4 Gy and in both the culture medium was changed immediately after irradiation, after 1 hour of recovery in fresh medium in standard growth conditions medium containing factors released by irradiated cells was collected from one flask, filtered from cell debris and untreated control K 562 cells were grown in this medium in standard conditions for 36h. The irradiated cells of the second flask were incubated for 36 hours in parallel and RNA was isolated from both irradiated and Irradiation Conditioned Medium-exposed samples. The transcript levels were measured in these RNA samples using Affymetrix HGU 133A microarrays and compared to the levels in RNA from control cells grown for 36h or control cells collected after 1h after change of medium. Experiments were repeated twice.

2009-12-30 | E-MEXP-1786 | biostudies-arrayexpress

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