Dataset Information

Mass spectrometry analysis of estrogen receptor α -interacting proteins.

ABSTRACT: Quantitative mass spectrometry analysis using tandem mass tags (TMT) labelling of estrogen receptor α (ERα) pull downs. Co-immunoprecipitated proteins using a anti Erα antibody were compared quantitatively with material recovered in a mock to identify ERα-interacting proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Juan Oses-Prieto

LAB HEAD: Davide Ruggero

PROVIDER: PXD025018 | Pride | 2022-10-14

REPOSITORIES: Pride

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Publications

ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.

Xu Yichen Y Huangyang Peiwei P Wang Ying Y Xue Lingru L Devericks Emily E Nguyen Hao G HG Yu Xiuyan X Oses-Prieto Juan A JA Burlingame Alma L AL Miglani Sohit S Goodarzi Hani H Ruggero Davide D

Cell 20210923 20

Estrogen receptor α (ERα) is a hormone receptor and key driver for over 70% of breast cancers that has been studied for decades as a transcription factor. Unexpectedly, we discover that ERα is a potent non-canonical RNA-binding protein. We show that ERα RNA binding function is uncoupled from its activity to bind DNA and critical for breast cancer progression. Employing genome-wide cross-linking immunoprecipitation (CLIP) sequencing and a functional CRISPRi screen, we find that ERα-associated mRN ...[more]

PMID: 34559986

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			Action	DRS
	ERaIP-searchresults.xlsx	Xlsx
	P20160914-10.raw	Raw
	P20160914-10_FTMSms2hcd.txt	Txt
	checksum.txt	Txt
	results-ERaIP-TMT-quantitiveresults.xlsx	Xlsx