Dataset Information

Chemical and Random Additive Noise Elimination (CRANE) - Case Study - Matrisome Dataset

ABSTRACT: The effectiveness of the novel electrospray ionisation - liquid chromatography mass spectrometry (ESI-LC-MS) data de-noising technique, CRANE, is demonstrated by denoising the MS1 and all the MS2 windows of a matrisome, data-independent acquisi-tion (DIA) MS dataset from Krasny, et al. (2018).

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Lung, Liver

SUBMITTER: Akila Seneviratne

LAB HEAD: Qing Zhong

PROVIDER: PXD025103 | Pride | 2021-07-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20171107_SWATH_LungE_3_R1.crane_V3_MS1_00001066_MS2_00001066.tof	Other
	20171107_SWATH_LungE_3_R1.tof	Other
	20171107_SWATH_LungE_3_R2.crane_V3_MS1_00001066_MS2_00001066.tof	Other
	20171107_SWATH_LungE_3_R2.tof	Other
	20171107_SWATH_LungE_3_R3.crane_V3_MS1_00001066_MS2_00001066.tof	Other

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Publications

SWATH mass spectrometry as a tool for quantitative profiling of the matrisome.

Krasny Lukas L Bland Philip P Kogata Naoko N Wai Patty P Howard Beatrice A BA Natrajan Rachael C RC Huang Paul H PH

Journal of proteomics 20180301

Proteomic analysis of extracellular matrix (ECM) and ECM-associated proteins, collectively known as the matrisome, is a challenging task due to the inherent complexity and insolubility of these proteins. Here we present sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH MS) as a tool for the quantitative analysis of matrisomal proteins in both non-enriched and ECM enriched tissue without the need for prior fractionation. Utilising a spectral library co ...[more]

PMID: 29501709

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