Proteomics

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Defining changes in the total levels of proteins during aging of C. elegans

ABSTRACT: We performed label-free proteomics in C. elegans to define and quantify changes in the ubiquitinated (Ub)-proteome during the aging process. More specifically, we compared wild-type worms at the first day of adulthood with young (day 5), mid-age (day 10) and aged adults (day 15). Moreover, we assessed protein levels of age-matched long-lived genetic models of dietary restriction (eat-2(ad1116)) and reduced insulin/IGF-1 signaling (daf-2(e1370)).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: David Vilchez  

LAB HEAD: David Vilchez

PROVIDER: PXD025128 | Pride | 2021-05-26

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
Proteins_ProjID_773_1316_Seda_Report_b.txt Txt
_N2-D1-1__20171122_Q4_JK_ColID_466_ProjID_773_Vilchez_sample_5.raw Raw
_N2-D1-2__20171128_Q4_JK_ColID_466_ProjID_773_Vilchez_sample_6.raw Raw
_N2-D1-3__20171129_Q4_JK_ColID_466_ProjID_773_Vilchez_sample_7.raw Raw
_N2-D1-4__20171201_Q4_JK_ColID_466_ProjID_773_Vilchez_sample_8.raw Raw
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Publications

Rewiring of the ubiquitinated proteome determines ageing in C. elegans.

Koyuncu Seda S   Loureiro Rute R   Lee Hyun Ju HJ   Wagle Prerana P   Krueger Marcus M   Vilchez David D  

Nature 20210728 7871

Ageing is driven by a loss of cellular integrity<sup>1</sup>. Given the major role of ubiquitin modifications in cell function<sup>2</sup>, here we assess the link between ubiquitination and ageing by quantifying whole-proteome ubiquitin signatures in Caenorhabditis elegans. We find a remodelling of the ubiquitinated proteome during ageing, which is ameliorated by longevity paradigms such as dietary restriction and reduced insulin signalling. Notably, ageing causes a global loss of ubiquitinatio  ...[more]

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