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EAE Optic Nerves Injected with LPC 18:1 + Isobaric C13-His

ABSTRACT: EAE mice were injected with LPC 18:1 and Isobaric C13-His at the optic nerve when they exhibited a clinical score of 2. Visual function was assessed via pattern electroretinogram and optic nerves were harvested 12 days post optic nerve injection. Optic nerves were processed for mass spectrometry to identify the integration of C13-His in newly synthesized proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Optic Nerve

DISEASE(S): Multiple Sclerosis

SUBMITTER: Sanjoy Bhattacharya

LAB HEAD: Sanjoy Bhattacharya

PROVIDER: PXD025176 | Pride | 2022-02-17

REPOSITORIES: Pride

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			Action	DRS
	C007-LPC-C13.mzML	Mzml
	C007-LPC-C13.mzid.gz	Mzid
	C007-LPC-C13.raw	Raw
	C007_2B_R_C13.mzML	Mzml
	C007_2B_R_C13.mzid.gz	Mzid

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Publications

Lyso-Lipid-Induced Oligodendrocyte Maturation Underlies Restoration of Optic Nerve Function.

Valdivia Anddre Osmar AO Bhattacharya Sanjoy K SK

eNeuro 20220126 1

Protein hyperdeimination and deficiency of lyso-phospholipids (LPC 18:1) has been associated with the pathology of demyelinating disease in both humans and mice. We uncovered interesting biology of LPC 18:1, in which LPC 18:1 induced optic nerve function restoration through oligodendrocyte maturation and remyelination in mouse model systems. Our <i>in vitro</i> studies show LPC 18:1 protection against neuron-ectopic hyperdeimination and stimulation of oligodendrocyte maturation, while <i>in vivo ...[more]

PMID: 35027445

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EAE Optic Nerve Injections with LPC 18:1 (iTRAQ)

Project description:EAE mice were injected with LPC 18:1 at the optic nerve when they exhibited a clinical score of 2. Visual function was assessed via pattern electroretinogram and optic nerves were harvested 12 days post optic nerve injection. Three different visual recovery pathways were observed: 1) No-injection control (no recovery in visual function), 2) Low recovery visual function, 3) High recovery visual function and 4) No change in visual function.

2022-02-17 | PXD024966 | Pride

OPC Cell Culture with LPC 18:1 or LPC 18:1 (both + C13-His)

Project description:Rat OPCs were incubated in the presence of LPC 18:1 or LPC 18:0 and C13-His. Cultures controls included no treatment and a positive control for the differentiation of OPCs. LPC 18:1 was administered at 10 uM while C13-His was administered at 10 uM. Samples were process for mass spectrometry to identify the incorporation of C13-His in newly synthesized proteins.

2022-02-17 | PXD025177 | Pride

Expression data from P4 and P10 mouse optic nerves

Project description:Optic nerves are an accessible part of the CNS, providing a source of glia without the presence of neuronal cell bodies. Therefore, an analysis was carried out of gene expression in optic nerves at P4, before myelination begins and at P10, when myelination is very actively proceeding. The goal was to obtain a profile of the changing gene expression that accompanies this transition from unmyelinated CNS nerve to myelinated nerve. Two microarray experiments were combined here. In the first, RNA was prepared from the optic nerves of 75 P4 C57BL/6J mice and 70 P10 C57BL/6J mice. In the second experiment, RNA was prepared from the optic nerves of 55 P4 C57BL/6J mice and two groups of 50 P10 C57Bl/6J mice. In total, there are 2 P4 RNA samples and 3 P10 RNA samples being hybridized to Affymetrix GeneChip 430A for Mouse Expression.

2011-05-16 | E-GEOD-24513 | biostudies-arrayexpress

Expression data from P4 and P10 mouse optic nerves

2011-05-16 | GSE24513 | GEO

Labeled quantitative proteomics dataset of optogenetics induced axon regeneration

Project description:This labeled quantitative proteomics dataset was collected from a transgenic channel rhodopsin mouse model (Chr2) subjected to light stimulation after traumatic optic nerve crush (ONC) was performed. Mouse models expressing channel rhodopsin were subjected to therapeutic light stimulation promoting axon regeneration of retinal ganglion cell (RGC) axons post optic nerve crush. Experimental mice and wild type control mice were euthanized, and optic nerves were collected. A protein extraction was carried out by careful mincing of the tissue in extraction buffer (TEAB, NaCl and SDS). Protein amount normalization across samples was achieved using dot blot densitometry using ImageJ software. Samples were labelled using a modified 16plex TMT (Tandem Mass Tag) kit for quantification after overnight trypsin digestion. Untargeted liquid chromatography-mass spectrometry was performed on an Easy-nLC 1000 liquid chromatograph coupled to a QExactive mass spectrometer (LC-MS/MS). Data analysis was performed using Proteome Discoverer 2.5.

2022-08-12 | PXD032788 | Pride

Retinal whole genome microarray analysis after irradiated injury of intraorbital nerve in the monkey

Project description:To obtain and analyze early retinal changes at molecular level at 24h after ipsilateral intraorbital nerve radiation injury by gamma knife surgery Unilateral intraorbital optic nerves of three Rhesus Macaques were treated by gamma knife surgery (GKS) with irradiated doses of 15Gy, while the contralateral optic nerves and retinas served as the control. Gene expression profiles of control and affected retinas were analyzed with Affymetrix Rhesus Macaque Genome arrays at 24h after treatment

2011-09-29 | E-GEOD-29901 | biostudies-arrayexpress

Nervous system injury establishes a microenvironment sufficient for brain tumor formation

Project description:To investigate the mechanism by which optic nerve crush induces microglia Ccl5 production, we performed bulk RNA sequencing on isolated optic nerves from crush- and sham-treatedNf1flox/flox;hGFAP-Cre miceat 3 months of age.

2024-02-07 | GSE233373 | GEO

Expression data for effect of LY294002 on White Matter

Project description:Characterisation of the effect of the PI3K/AKT modulator LY294002 in the murine optic nerve. Microarray analysis was performed using the Affymetrix Genechip Mouse 430 2.0 on murine optic nerves to identify bipartite effect of LY294002 on glia

2022-01-10 | GSE179189 | GEO

Expression data from mouse optic nerve head after optic nerve crush

Project description:Reactive gliosis is a complex process that involves profound changes in gene expression. We used microarray to indentify differentially expressed genes and to investigate the molecular mechanisms of reactive gliosis in optic nerve head in response to optic nerve crush injury. C57Bl/6 female mice were 6-8 weeks old at the time of optic nerve crush surgery. The optic nerve in the left eye was crush 1 mm behind the globe for 10 seconds and the right eye served as contralateral control. The animals were allowed to recover for 1 day, 3 day, 1 week, 3 weeks and 3 months before the optic nerve heads were collected. The naive control mice did not receive any surgery in either eye. Due to the small tissue size of the mouse optic nerve head, two optic nerve heads were pooled together for each microarray chip. The left eyes and the right eyes of two mice were combined respectively to form one pair of experiment and control samples. There were five biological replicates (10 mice) for each condition.

2013-07-15 | E-GEOD-40857 | biostudies-arrayexpress

The proteome of human retrobulbar optic nerve

Project description:The optic nerve is a white matter tract that conveys visual information to the brain. A detailed investigation of the proteome of the normal human retrobulbar optic nerve may help facilitate studies of the biology and pathophysiology of the optic nerve. We conducted an in-depth proteomic analysis of optic nerve from five adults. Proteins were fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. We identified 2,711 non-redundant proteins in the human retrobulbar optic nerve, including the astrocytic marker glial fibrillary acidic protein, several proteins expressed by oligodendrocytes (laminin, proteolipid protein, and fibronectin), myelin proteins (myelin basic protein, myelin-associated glycoprotein), paranodal structural proteins (neurofascin, contactin, α, β, and γ adducins, septin 2, endophilin, ankyrin β, spectrin), proteins involved in neuronal protection and regeneration (α crytallins A and B, dedicator of cytokinesis proteins, ciliary neurotrophic factor), proteins associated with open-angle glaucoma (thioredoxin, heat shock protein-70), and proteins associated with optic neuritis (aquaporin-4). Twenty-one unambiguous protein isoforms were identified in the optic nerve.

2016-08-24 | PXD001581 | Pride

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