Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

To the islands their own: Venomics of the enigmatic Andaman cobra (N. sagittifera) and the preclinical failure of Indian antivenoms in Andaman and Nicobar Islands

ABSTRACT: In this study, we unveil the venom composition, biochemistry, pharmacological activity, and potency of the Andaman cobra (N. sagittifera).

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Naja Naja Naja Sagittifera

TISSUE(S): Venom

SUBMITTER: Kartik Sunagar

LAB HEAD: Dr. Kartik Sunagar

PROVIDER: PXD025362 | Pride | 2022-02-17

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	NaNaKA16.pep.xml	Pepxml
	NaNaKA16_F1.raw	Raw
	NaNaKA16_F10.raw	Raw
	NaNaKA16_F11.raw	Raw
	NaNaKA16_F12.raw	Raw

Items per page:

1 - 5 of 30

Publications

Venomics of the Enigmatic Andaman Cobra (Naja sagittifera) and the Preclinical Failure of Indian Antivenoms in Andaman and Nicobar Islands.

Attarde Saurabh S Khochare Suyog S Iyer Ashwin A Dam Paulomi P Martin Gerard G Sunagar Kartik K

Frontiers in pharmacology 20211025

The Andaman and Nicobar Islands are an abode to a diversity of flora and fauna, including the many endemic species of snakes, such as the elusive Andaman cobra (Naja sagittifera). However, the ecology and evolution of venomous snakes inhabiting these islands have remained entirely uninvestigated. This study aims to bridge this knowledge gap by investigating the evolutionary history of N. sagittifera and its venom proteomic, biochemical and toxicity profile. Phylogenetic reconstruct ...[more]

PMID: 34759827

Similar Datasets

The Arabian cousin: Comparative venomics of Daboia palaestinae and Daboia russelii

Project description:In this study, to unravel the influence of phylogenetic divergence and biogeography in shaping the composition and activity of Daboia venoms, we comparatively investigated the venoms of D. russelii from western India and D. palaestinae from Israel.

2023-03-11 | PXD031190 | Pride

Proteomic Investigations of Two Pakistani Naja Snake Venoms Species Unravel the Venom Complexity, Posttranslational Modifications and Presence of Extracellular Vesicles

Project description:Latest advancement of omics technologies allows in-depth characterization of venom compositions. In the present work we present a proteomic study of two snake venoms of the genus Naja i.e. Naja naja (black cobra) and Naja oxiana (brown cobra), of Pakistani origin. The present study has shown that these snake venoms consist of a highly diversified proteome. Furthermore, the data also revealed variation among closely related species. High throughput mass spectrometric analysis of the venom proteome allowed to identify for the N. naja venom 34 protein families and for the N. oxiana 24 protein families. The comparative evaluation of the two venoms showed that N. naja consists of a more complex venom proteome than N. oxiana venom.

2020-10-29 | PXD018726 | Pride

Venomics of the desert black cobra Walterinnesia aegyptia from Riyadh, SA

Project description:Combined transcriptomics-assisted bottom-up and top-down MS characterization of the venom proteome of the desert black cobra Walterinnesia aegyptia from Riyadh, SA

2022-02-17 | PXD027498 | Pride

Venomics of the desert black cobra Walterinnesia aegyptia from Sinai Peninsula, Egypt

Project description:Combined transcriptomics-assisted bottom-up and top-down MS characterization of the venom proteome of the desert black cobra Walterinnesia aegyptia from Sinai Peninsula, Egypt.

2022-02-17 | PXD027495 | Pride

Venomics of the desert black cobra Walterinnesia morgani from Çörten village, Turkey

Project description:Combined transcriptomics-assisted bottom-up and top-down MS characterization of the venom proteome of the desert black cobra Walterinnesia morgani from Çörten village, Turkey

2022-02-17 | PXD027497 | Pride

Evaluation of antifungal activity of Naja pallida and Naja mossambica venoms against three Candida species

Project description:In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra Naja pallida (NPV) and Mozambique spitting cobra Naja mossambica (NMV) was characterized using LC-MS/MS approach and the biological activity of crude venoms against three Candida species was established.

2020-08-12 | PXD020450 | Pride

Top-down venomics Mapping proteoforms and protein complexes from king cobra venom

Project description:Characterization of King cobra venom by denaturing and native top-down proteomics.

2015-10-18 | MSV000079351 | MassIVE

Venomics of the Enigmatic Andaman Cobra (Naja sagittifera) and the Preclinical Failure of Indian Antivenoms in Andaman and Nicobar Islands.

Project description:The Andaman and Nicobar Islands are an abode to a diversity of flora and fauna, including the many endemic species of snakes, such as the elusive Andaman cobra (Naja sagittifera). However, the ecology and evolution of venomous snakes inhabiting these islands have remained entirely uninvestigated. This study aims to bridge this knowledge gap by investigating the evolutionary history of N. sagittifera and its venom proteomic, biochemical and toxicity profile. Phylogenetic reconstructions confirmed the close relationship between N. sagittifera and the Southeast Asian monocellate cobra (N. kaouthia). Overlooking this evolutionary history, a polyvalent antivenom manufactured using the venom of the spectacled cobra (N. naja) from mainland India is used for treating N. sagittifera envenomations. Comparative evaluation of venoms of these congeners revealed significant differences in their composition, functions and potencies. Given the close phylogenetic relatedness between N. sagittifera and N. kaouthia, we further assessed the cross-neutralising efficacy of Thai monovalent N. kaouthia antivenom against N. sagittifera venoms. Our findings revealed the inadequate preclinical performance of the Indian polyvalent and Thai monovalent antivenoms in neutralising N. sagittifera venoms. Moreover, the poor efficacy of the polyvalent antivenom against N. naja venom from southern India further revealed the critical need to manufacture region-specific Indian antivenoms.

| S-EPMC8573199 | biostudies-literature

Denaturing and native top-down venomics: Mapping proteoforms and protein complexes from king cobra venom

Project description:Characterizing whole proteins by top-down proteomics avoids a step of inference encountered in the dominant bottom-up methodology when peptides are assembled computationally into proteins for identification. The direct interrogation of whole proteins and protein complexes from the venom of Ophiophagus hannah (king cobra) provides a sharply clarified view of toxin sequence variation, transit peptide cleavage sites and post-translational modifications (PTMs) likely critical for venom lethality. A tube-gel format for electrophoresis (called GELFrEE) and solution isoelectric focusing were used for protein fractionation prior to LC-MS/MS analysis resulting in 131 protein identifications (18 more than bottom-up) and a total of 184 proteoforms characterized from 14 protein toxin families. Operating both GELFrEE and mass spectrometry to preserve non-covalent interactions generated detailed information about two of the largest venom glycoprotein complexes: the homodimeric L-amino acid oxidase (LAAO, ~130 kDa) and the multi-chain toxin cobra venom factor (~147 kDa). The LAAO complex exhibited two clusters of multi-proteoform complexes corresponding to the presence of 5 or 6 N-glycans moieties, each consistent with a distribution of N-acetyl hexosamines. Employing top-down proteomics in both native and denaturing modes provides unprecedented characterization of venom proteoforms and their complexes. A precise molecular inventory of venom proteins will propel the study of snake toxin variation and the targeted development of new anti-venoms or other biotherapeutics.

2016-05-18 | PXD003403 | Pride

The preclinical evaluation of a second-generation antivenom for treating snake envenoming in India

Project description:In this study, by employing chromatographic purification of the bulk, we have significantly enhanced the preclinical performance of the conventional Indian antivenom product. The effectiveness of the test batches of this ‘second-generation’ antivenom was evaluated using a variety of in vitro and in vivo preclinical assays, including venom recognition and toxicity and pathology neutralisation. The outcomes of these experiments demonstrated the significantly superior performance of the purified product over all other major commercial Indian antivenoms.

2022-04-04 | PXD029436 | Pride