PfCDPK7 signaling regulates phospholipid biogenesis in Plasmodium falciparum
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ABSTRACT: Calcium Dependent Protein Kinases (CDPKs) play important function in calcium signaling at various stage of Plasmodium falciparum. CDPK7 is one such kinase, which plays a crucial role in the development of Plasmodium falciparum parasite. In order to understand the function of PfCDPK7, we performed comparative phosphoproteomic analysis of PfCDPK7 knockout mutant and wild-type lines. In brief, proteins from PfCDPK7 knockout mutant and wild-type parasites were isolated, digested with trypsin and labelled with iTRAQ tags. Phosphopeptides were then enriched from iTRAQ labeled peptides and subjected to LC-MS/MS analysis. This led to the identification of putative substrates of PfCDPK7 in the parasites. A comparative proteomic analysis was performed to measure the protein abundance changes in the PfCDPK7 mutant parasites.
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Plasmodium Falciparum (isolate 3d7)
SUBMITTER: Keshava Prasad T. S.
LAB HEAD: T. S. Keshava Prasad
PROVIDER: PXD025384 | Pride | 2021-11-08
REPOSITORIES: Pride
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