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FASP analysis of Drosophila tissue

ABSTRACT: Drosophila testes tissue from WT and two different KO models (GC17669 and GC6980) subjected to standar FASP protocol, to analyse changes in expression levels of cilia related proteins.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Testis

SUBMITTER: Alfonso Bolado

LAB HEAD: Alexander von Kriegsheim

PROVIDER: PXD025409 | Pride | 2021-11-03

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	L_CG17669_1.raw	Raw
	L_CG17669_2.raw	Raw
	L_CG17669_3.raw	Raw
	L_CG17669_4.raw	Raw
	L_CG6980_1.raw	Raw

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Publications

The Drosophila orthologue of the primary ciliary dyskinesia-associated gene, DNAAF3, is required for axonemal dynein assembly.

Zur Lage Petra P Xi Zhiyan Z Lennon Jennifer J Hunter Iain I Chan Wai Kit WK Bolado Carrancio Alfonso A von Kriegsheim Alex A Jarman Andrew P AP

Biology open 20211028 10

Ciliary motility is powered by a suite of highly conserved axoneme-specific dynein motor complexes. In humans, the impairment of these motors through mutation results in the disease primary ciliary dyskinesia (PCD). Studies in Drosophila have helped to validate several PCD genes whose products are required for cytoplasmic pre-assembly of axonemal dynein motors. Here we report the characterisation of the Drosophila orthologue of the less-known assembly factor DNAAF3. This gene, CG17669 (Dnaaf3), ...[more]

PMID: 34553759

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