Proteomics

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Unbiased quantitative analysis of the cardiac phosphoproteome in response to acute β-adrenergic receptor stimulation in vivo

ABSTRACT: We utilised Super-SILAC mouse technology to perform an unbiased, quantitative analysis of the cardiac phosphoproteome in mice acutely treated in vivo with either saline or isoprenaline (ISO), a non-selective β-AR agonist. Using a customized mass spectrometry-based workflow (Figure 1), our study identified hundreds of phosphosites that are significantly regulated by β-AR stimulation, including both novel phosphoproteins and phosphosites that have not been previously identified as downstream effectors of β-AR stimulation and established downstream components of cAMP/PKA and CaMKII signaling pathways. Interestingly, our study revealed both up- and down-phosphorylation in comparable numbers, indicative of an intricate signaling network activated in response to β-AR stimulation.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Christof Lenz  

LAB HEAD: Christof Lenz

PROVIDER: PXD025569 | Pride | 2021-12-30

REPOSITORIES: Pride

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Publications

Quantitative Analysis of the Cardiac Phosphoproteome in Response to Acute β-Adrenergic Receptor Stimulation In Vivo.

Güran Alican A   Ji Yanlong Y   Fang Pan P   Pan Kuan-Ting KT   Urlaub Henning H   Avkiran Metin M   Lenz Christof C  

International journal of molecular sciences 20211122 22

β-adrenergic receptor (β-AR) stimulation represents a major mechanism of modulating cardiac output. In spite of its fundamental importance, its molecular basis on the level of cell signalling has not been characterised in detail yet. We employed mass spectrometry-based proteome and phosphoproteome analysis using SuperSILAC (spike-in stable isotope labelling by amino acids in cell culture) standardization to generate a comprehensive map of acute phosphoproteome changes in mice upon administration  ...[more]

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