Dataset Information

The impact of Zika virus exposure on the placental proteomic profile

ABSTRACT: Zika virus (ZIKV) infection has caused severe unexpected clinical outcomes in neonates and adults during the recent outbreak in Latin America, particularly in Brazil. Congenital malformations associated with ZIKV have been frequently reported; nevertheless, the mechanism of vertical transmission and the involvement of placental cells remains unclear. In this study, we applied quantitative proteomics analysis in a floating explant model of chorionic villi of human placental tissues incubated with ZIKV and with ZIKV pre-adsorbed with anti-ZIKV envelope protein. The regulation of specific proteins was measured using immunofluorescence and immunoperoxidase assays. Altered levels of proteins were involved in cell proliferation, apoptosis, inflammatory processes, and the integrin-cytoskeleton complex. Antibody-opsonized ZIKV particles differentially modulated the pattern of protein expression in placental cells; this phenomenon may play a pivotal role in determining the course of infection and the role of mixed infections. These data fill gaps in our understanding of ZIKV in the placenta and help identify infection control targets.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Placenta

DISEASE(S): Zika Virus Congenital Syndrome,Zika Fever

SUBMITTER: Giuseppe Palmisano

LAB HEAD: Estela M. A. F. Bevilacqua

PROVIDER: PXD025764 | Pride | 2021-12-30

REPOSITORIES: Pride

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			Action	DRS
	LUM2_00566_LR_OT-IT_ZIKV_AB_1.raw	Raw
	LUM2_00567_LR_OT-IT_ZIKV_AB_2.raw	Raw
	LUM2_00568_LR_OT-IT_ZIKV_AB_3.raw	Raw
	LUM2_00569_LR_OT-IT_ZIKV_1.raw	Raw
	LUM2_00570_LR_OT-IT_ZIKV_2.raw	Raw

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The impact of Zika virus exposure on the placental proteomic profile.

Rosa-Fernandes Livia L Bandeira Carla C Pour Shahab Zaki SZ Benedetti Viviane de Fátima VF Ferreira Daniel D Lorenzon Aline R AR Moreli Jusciele B JB Marinho Claudio Romero Farias CRF Larsen Martin R MR Zanotto Paolo P Palmisano Giuseppe G Bevilacqua Estela E

Biochimica et biophysica acta. Molecular basis of disease 20210925 1

Zika virus (ZIKV) infection has caused severe unexpected clinical outcomes in neonates and adults during the recent outbreak in Latin America, particularly in Brazil. Congenital malformations associated with ZIKV have been frequently reported; nevertheless, the mechanism of vertical transmission and the involvement of placental cells remains unclear. In this study, we applied quantitative proteomics analysis in a floating explant model of chorionic villi of human placental tissues incubated with ...[more]

PMID: 34582966

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