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Proteomic analysis of induced pluripotent stem cell-derived kidney organoids

ABSTRACT: Kidney organoids were generated from a control iPSC line using a previously published protocol (https://doi.org/10.1038/nprot.2016.098). Organoids were collected at three timepoints during the protocol (day 14, 18 and 25) and prepared for proteomic analyses. The focus of the study was to define the proteomic composition of kidney organoids during differentiation with a particular emphasis on the extracellular matrix and its comparison to in vivo systems. Following a ample fractionation and matrix enrichment strategy, samples were prepared for high resolution label-free tandem mass spectrometry to define the proteomic composition of human kidney organoids.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Louise Hopkinson

LAB HEAD: Rachel Lennon

PROVIDER: PXD025838 | Pride | 2021-10-13

REPOSITORIES: Pride

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			Action	DRS
	20200116_LennonR_TianP_01.raw	Raw
	20200116_LennonR_TianP_02.raw	Raw
	20200116_LennonR_TianP_03.raw	Raw
	20200116_LennonR_TianP_04.raw	Raw
	20200116_LennonR_TianP_05.raw	Raw

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