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Trifunctional PIP3 binder identification

ABSTRACT: Trifunctional PIP3 is a chemically modified derivate of PIP3 that includes 3 functionalized groups allowing spacial an temporal manipulation of its release and function. A caged group allows for timed release of the coumpound, a cross-linking group induces covalent binding to binders, while a clickable group is used for specific pull=out using functionilized beads. These functionalities comibend create a precise discovery tool used to detect PIP3 interactors.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Ana Kojic

LAB HEAD: Carsten Schultz

PROVIDER: PXD025903 | Pride | 2025-02-19

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	P1277_PH_AK.sf3	Other
	P1351_PH_AK.sf3	Other
	Zelda_200605_P1277_MR_AK_-UV_C_1out10_R1.raw	Raw
	Zelda_200605_P1277_MR_AK_-UV_M_1out10_R1.raw	Raw
	Zelda_200605_P1277_MR_AK_K_C_1out10_R1.raw	Raw

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Publications

Synthesis and Cellular Labeling of Multifunctional Phosphatidylinositol Bis- and Trisphosphate Derivatives.

Müller Rainer R Kojic Ana A Citir Mevlut M Schultz Carsten C

Angewandte Chemie (International ed. in English) 20210726 36

We synthesized the first multifunctionalized phosphoinositide polyphosphate derivatives featuring a photo-removable protecting group ("cage"), a photo-crosslinkable diazirine group, and a terminal alkyne group useful for click chemistry. We demonstrate that the lipid derivatives readily enter cells. After photo-crosslinking, cell fixation and fluorescent tagging via click chemistry, we determined the intracellular location of the lipid derivatives before and after uncaging of the lipids. We find ...[more]

PMID: 34075669

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