Dataset Information

Proteomics characterisation of induced pluripotent stem cells and cortical brain organoids.

ABSTRACT: A method was developed to reproducibly produce neural retina and cortical brain regions from confluent cultures of stem cells. The spontaneously generated cortical organoids were isolated and cultured in suspension conditions for maturation. Proteomic analysis of both the original induced pluripotent stem cells and the cortical organoids demonstrated the increased presence of synaptic components, indicating maturity.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pluripotent Stem Cell, Brain, Neural Retina

SUBMITTER: Mark Graham

LAB HEAD: Mark Evan Graham

PROVIDER: PXD025933 | Pride | 2022-05-06

REPOSITORIES: Pride

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			Action	DRS
	20200504-JW378-JKCMRI_18_BPhys-s22_f16_19.raw	Raw
	20200504-JW378-JKCMRI_18_BPhys-s22_f20_22.raw	Raw
	20200504-JW378-JKCMRI_18_BPhys-s22_f23.raw	Raw
	20200504-JW378-JKCMRI_18_BPhys-s22_f24.raw	Raw
	20200504-JW378-JKCMRI_18_BPhys-s22_f25.raw	Raw

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Publications

Differentiation of brain and retinal organoids from confluent cultures of pluripotent stem cells connected by nerve-like axonal projections of optic origin.

Fernando Milan M Lee Scott S Wark Jesse R JR Xiao Di D Lim Benjamin Y BY O'Hara-Wright Michelle M Kim Hani J HJ Smith Grady C GC Wong Ted T Teber Erdahl T ET Ali Robin R RR Yang Pengyi P Graham Mark E ME Gonzalez-Cordero Anai A

Stem cell reports 20220505 6

Advances in the study of neurological conditions have been possible because of pluripotent stem cell technologies and organoids. Studies have described the generation of neural ectoderm-derived retinal and brain structures from pluripotent stem cells. However, the field is still troubled by technical challenges, including high culture costs and variability. Here, we describe a simple and economical protocol that reproducibly gives rise to the neural retina and cortical brain regions from conflue ...[more]

PMID: 35523177

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