Proteomics

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A homeobox7 transcription factor from Mesembryanthemum crystallinum enhanced salt tolerance


ABSTRACT: Mesembryanthemum crystallinum (common ice plant) is a facultative halophyte species, which has adapted to extreme conditions. In this study, we cloned a McHB7 transcription factor gene from the ice plant. The expression of McHB7 was significantly induced by 500 mM NaCl and it reached the peak under salt treatment for 7 days. The McHB7 protein was targeted to the nucleus. Overexpression of McHB7 gene in ice plant leaves by Agrobacterium-mediated transformation led to 25 times more McHB7 transcripts than the non-transformed control wild type (WT). After 500 mM NaCl treatment for 7 days, SOD, POD activities and water content of the transgenic plants were significantly higher than WT, while MDA content was decreased in the transgenic plants. Proteomics results showed that a total of 1082 and 1072 proteins were profiled under control and salt treatment, respectively. 22 (2%) and 11 (1%) proteins were uniquely identified under control and salt stress conditions, respectively. Among these 11 proteins, 7 were increased and 4 were decreased. Most identified proteins involved in the processes of regulation of biological, transporter and catalytic activity, biosynthesis of secondary metabolties and response to stimulus were significantly increased in the McHB7 overexpression ice plants under high salinity. All the results demonstrate that the McHB7 transcription factor plays a positive role in improving plant salt tolerance.

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Mesembryanthemum Crystallinum (common Ice Plant) (cryophytum Crystallinum)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Xuemei Zhang  

LAB HEAD: Sixue Chen

PROVIDER: PXD025961 | Pride | 2022-02-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CM1-_3_.msf Msf
CM1-_3_.pdResultView Other
OEC1.raw Raw
OEC2.raw Raw
OEC3.raw Raw
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Publications

Proteomics of Homeobox7 Enhanced Salt Tolerance in <i>Mesembryanthemum crystallinum</i>.

Zhang Xuemei X   Tan Bowen B   Zhu Dan D   Dufresne Daniel D   Jiang Tingbo T   Chen Sixue S  

International journal of molecular sciences 20210615 12


<i>Mesembryanthemum crystallinum</i> (common ice plant) is a halophyte species that has adapted to extreme conditions. In this study, we cloned a <i>McHB7</i> transcription factor gene from the ice plant. The expression of <i>McHB7</i> was significantly induced by 500 mM NaCl and it reached the peak under salt treatment for 7 days. The <i>McHB7</i> protein was targeted to the nucleus. <i>McHB7</i>-overexpressing in ice plant leaves through <i>Agrobacterium</i>-mediated transformation led to 25 t  ...[more]

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