Dataset Information

Quantitative Proteomic and Phosphoproteomic profilings of two foxtail millet cultivars under salinity

ABSTRACT: In this project, comprehensive proteomic and phosphoproteomic analysis was performed to explore sophisticated responsive networks of foxtail millet varieties of An04 and Yugu2 under salinity. Isobaric tags for relative and absolute quantitation-based (iTRAQ) and tandem mass tags-based (TMTs) quantitative proteomics approaches were used. In total, 10366 sites corresponding to 2862 proteins were detected and quantified. There were 759 and 990 sites corresponding to 484 and 633 proteins identified under salinity in An04 and Yugu2, respectively, and 1264 and 1131 phosphorylation sites corresponding to 789 and 731 proteins were identified between these two varieties before and after salt stress, respectively.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Setaria Italica

TISSUE(S): Root

SUBMITTER: li zhen

LAB HEAD: Liu Wei

PROVIDER: PXD025969 | Pride | 2022-02-17

REPOSITORIES: Pride

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Publications

Phosphoproteomic Profiling Reveals Early Salt-Responsive Mechanisms in Two Foxtail Millet Cultivars.

Pan Jiaowen J Li Zhen Z Wang Qingguo Q Guan Yanan Y Li Xiaobo X Huangfu Yongguan Y Meng Fanhua F Li Jinling J Dai Shaojun S Liu Wei W

Frontiers in plant science 20210920

Excess soluble salts in saline soils are harmful to most plants. Understanding the biochemical responses to salts in plants and studying the salt tolerance-associated genetic resources in nature will contribute to the improvement of salt tolerance in crops. As an emerging model crop, foxtail millet (<i>Setaria italica</i> L.) has been regarded as a novel species for stress resistance investigation. Here, the dynamic proteomic and phosphoproteomic profiling of two foxtail millet varieties of An04 ...[more]

PMID: 34616412

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Project description:This batch of data is for three independent projects. Since in this three projects, the mouse strains are all CD1/ICR, the sex are both female, and the mouse ages are all two-month-old, three projects shared control data to save expenditure legitimately. But in our hand we couldn't segregate the raw data into two parts corresponding to two projects, so we uploads all raw data. However, we also provided researcher-analyzable excel files including the expression of all phosphorylated proteins. The concise description of these three projects are as follow: Project 1: We found that in Chronic Resistant Stress (CRS)-treated mice, the fertility and oocyte quality significantly decreased, and plenty of biochemical indexes significantly changed, so we guessed that the overall physical health of CRS mice was severely impacted. As one major strategy to investigate the mechanism how CRS impact the physical health, We chose TMT-labeled quantitative ovarian phosphoproteomics service from Hangzhou PTM Bio Co. We sent two repeats of two-month-old CRS CD1/ICR mouse ovaries and two repeats of control ovaries for characterization of differentially phosphorylated proteins. Each repeat contained about 60 ovaries from 30 female mice, about 300 mg. Project 2: We found that in High Salt (HS)-treated mice, the fertility and oocyte quality significantly decreased, and plenty of biochemical indexes significantly changed, so we guessed that the overall physical health of HS mice was severely impacted. As one major strategy to investigate the mechanism how HS impact the physical health, We chose TMT-labeled Quantitative ovarian phosphoproteomics service from Hangzhou PTM Bio Co. We sent two repeats of two-month-old HS CD1/ICR mouse ovaries and two repeats of control ovaries for characterization of differentially phosphorylated proteins. Each repeat contained about 60 ovaries from 30 female mice, about 300 mg. Project 3: We found that in arecoline (Are)-treated mice, the fertility and oocyte quality significantly decreased, and plenty of biochemical indexes significantly changed, so we guessed that the overall physical health of Are mice was severely impacted. As one major strategy to investigate the mechanism how Are impact the physical health, We chose TMT-labeled Quantitative ovarian phosphoproteomics service from Hangzhou PTM Bio Co. We sent two repeats of two-month-old Are CD1/ICR mouse ovaries and two repeats of control ovaries for characterization of differentially phosphorylated proteins. Each repeat contained about 60 ovaries from 30 female mice, about 300 mg. Two repeats of control samples and data were shared among project 1, 2 and 3. Gross protein levels were also examined and used to normalize the protein phosphorylation level.

			Action	DRS
	S8338TPST_rep1.zip	Other
	S8338TPST_rep1_1.raw	Raw
	S8338TPST_rep1_2.raw	Raw
	S8338TPST_rep1_3.raw	Raw
	S8338TPST_rep1_4.raw	Raw