Proteomics

Dataset Information

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Proximity-dependent biotinylation detects association between stress granule associated proteins and SARS coronavirus nonstructural protein 1


ABSTRACT: Severe acute respiratory syndrome coronavirus (SARS-CoV and SARS-CoV-2) nonstructural protein 1 (nsp1) is a critical viral protein that suppresses host gene expression by blocking the assembly of the ribosome on host messenger RNAs (mRNA). Using proximity-dependent biotinylation assay followed by proteomic analyses of biotinylated proteins, we captured multiple dynamic interactions of nsp1 with host cell proteins. In addition to ribosomal proteins, we have identified several mRNA processing proteins, including splicing factors and transcription termination proteins, exosomes- and stress granule (SG) associated proteins. The interactions with transcription termination factors are primarily governed by the C-terminal region of nsp1 and are disrupted by the mutation of K164 and H165 amino acids. We further show that nsp1 associates with SG protein G3BP and colocalizes with G3BP in SGs under sodium arsenite-induced stress for a short period of time. However, the presence of nsp1 disrupts the maturation of SGs over a long period.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Severe Acute Respiratory Syndrome

SUBMITTER: Jennifer Bethard  

LAB HEAD: Anita Nag

PROVIDER: PXD025980 | Pride | 2022-02-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
020420_SP_HUMAN-n20354.fasta Fasta
09052020_Nag_BirA_1.raw Raw
09052020_Nag_BirA_2.raw Raw
09052020_Nag_BirA_3.raw Raw
09052020_Nag_NCov_1.raw Raw
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Publications

Proximity-dependent biotinylation detects associations between SARS coronavirus nonstructural protein 1 and stress granule-associated proteins.

Gerassimovich Yevgeniy A YA   Miladinovski-Bangall Samantha J SJ   Bridges Kaitlin M KM   Boateng Linkel L   Ball Lauren E LE   Valafar Homayoun H   Nag Anita A  

The Journal of biological chemistry 20211111 6


The nonstructural protein 1 (nsp1) of severe acute respiratory syndrome coronavirus and severe acute respiratory syndrome coronavirus 2 is a critical viral protein that suppresses host gene expression by blocking the assembly of the ribosome on host mRNAs. To understand the mechanism of inhibition of host gene expression, we sought to identify cellular proteins that interact with nsp1. Using proximity-dependent biotinylation followed by proteomic analyses of biotinylated proteins, here we captur  ...[more]

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