Proteomics

Dataset Information

0

Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly


ABSTRACT: Cells respond to stress by blocking translation, rewiring metabolism, and forming transient mRNP assemblies called stress granules (SGs). After stress release, re-establishing homeostasis requires energy-consuming processes. However, the molecular mechanisms whereby cells restore energy production to disassemble SGs and reinitiate growth after stress remain poorly understood. Here we show that, upon stress, the ATP-producing enzyme Cdc19 forms inactive amyloids, and that their rapid re-solubilization is essential to restore energy production and SG disassembly. Cdc19 re-solubilization is initiated by the glycolytic metabolite fructose-1,6-bisphosphate (FBP), which directly binds Cdc19 amyloids and facilitates conformational changes that allow Hsp104 and Ssa2 chaperone recruitment. FBP then promotes Cdc19 tetramerization and activity, which together with trehalose breakdown boosts glycolysis to further enhance SG disassembly. Together, these results provide a molecular mechanism protecting cells during stress by directly coupling metabolism and ATP production with SG dynamics via regulation of Cdc19 amyloids.

INSTRUMENT(S): Q TRAP

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Ilaria Piazza  

LAB HEAD: Paola Picotti

PROVIDER: PXD026060 | Pride | 2021-08-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
180114_EXvsStar_wt_L.wiff Wiff
180114_EXvsStar_wt_L.wiff.scan Wiff
180114_EXvsStar_wt_T.wiff Wiff
180114_EXvsStar_wt_T.wiff.scan Wiff
180122_EXvsStar_4D_L.wiff Wiff
Items per page:
1 - 5 of 15

Similar Datasets

2023-10-11 | GSE223295 | GEO
2024-08-22 | PXD044922 | Pride
2015-02-27 | E-GEOD-65778 | biostudies-arrayexpress
2019-01-30 | GSE119977 | GEO
2019-10-03 | PXD014828 | Pride
2015-02-27 | GSE65778 | GEO
2019-11-01 | GSE138058 | GEO
2024-05-01 | GSE263848 | GEO
2021-09-30 | E-MTAB-9096 | biostudies-arrayexpress
2023-02-03 | GSE220821 | GEO