Dataset Information

Protein profiling of transport vesicles produced under different conditions

ABSTRACT: 1)Identification of cytosolic proteins that are associated with vesicles in a GTP dependent manner and cargo proteins that are packaged into vesicles in a GTP-hydrolysis dependent manner; 2) Identification of cargo proteins and cytosolic proteins that are dependent on Sar1A to be associated with transport vesicles; 3) Identification of cargo proteins that depend on a specific cargo receptor for packaging into transport vesicles

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Yusong Guo

LAB HEAD: Yusong Guo

PROVIDER: PXD026081 | Pride | 2022-02-17

REPOSITORIES: Pride

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Publications

An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.

Huang Yan Y Yin Haidi H Li Baiying B Wu Qian Q Liu Yang Y Poljak Kristina K Maldutyte Julija J Tang Xiao X Wang Mo M Wu Zhixiao Z Miller Elizabeth A EA Jiang Liwen L Yao Zhong-Ping ZP Guo Yusong Y

Proceedings of the National Academy of Sciences of the United States of America 20210801 35

The fidelity of protein transport in the secretory pathway relies on the accurate sorting of proteins to their correct destinations. To deepen our understanding of the underlying molecular mechanisms, it is important to develop a robust approach to systematically reveal cargo proteins that depend on specific sorting machinery to be enriched into transport vesicles. Here, we used an in vitro assay that reconstitutes packaging of human cargo proteins into vesicles to quantify cargo capture. Quanti ...[more]

PMID: 34433667

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