Dataset Information

Protein changes in the infarct region of ischemia/reperfused rat hearts revealed by spatialOMx

ABSTRACT: Myocardial infarction (MI) is the most common cause of death worldwide. Timely diagnosis and early restoration of the blood flow prevents further tissue damage. However, this sudden reperfusion can also lead to so-called ischemia/reperfusion (I/R) injury. Although the different pathological processes activated in I/R injury, like cell death and inflammation have been previously addressed, the exact pathophysiological mechanisms remain unclear. For the development of strategies that minimize myocardial damage a better understanding of the underlying alterations in protein and signaling pathways is required. In this study we aim to identify the protein signature of cardiac I/R injury, therefore we use a spatialOMx approach, combining protein mass spectrometry imaging (MSI) and a classical label-free proteomics workflow on the same tissue section, to further address the observed alterations

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart, Cardiac Muscle Cell

DISEASE(S): Myocardial Ischemia

SUBMITTER: Stephanie Mezger

LAB HEAD: Berta Cillero-Pastor

PROVIDER: PXD026115 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	201028_Steph_RatT3_sl1-6_IR1.raw	Raw
	201028_Steph_RatT3_sl1-6_IR2.raw	Raw
	201028_Steph_RatT3_sl1-6_IR4.raw	Raw
	201028_Steph_RatT3_sl3-6_IRL1.raw	Raw
	201028_Steph_RatT3_sl3-6_IRL3.raw	Raw

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Publications

Protein Alterations in Cardiac Ischemia/Reperfusion Revealed by Spatial-Omics.

Mezger Stephanie T P STP Mingels Alma M A AMA Soulié Matthieu M Peutz-Kootstra Carine J CJ Bekers Otto O Mulder Paul P Heeren Ron M A RMA Cillero-Pastor Berta B

International journal of molecular sciences 20221110 22

Myocardial infarction is the most common cause of death worldwide. An understanding of the alterations in protein pathways is needed in order to develop strategies that minimize myocardial damage. To identify the protein signature of cardiac ischemia/reperfusion (I/R) injury in rats, we combined, for the first time, protein matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) and label-free proteomics on the same tissue section placed on a conductive slide. Wistar ra ...[more]

PMID: 36430335

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