Dataset Information

Proteins that co-purify with tandem affinity-purified PRC5 in Trypanosoma brucei

ABSTRACT: The project was to determine the role of a trypanosomatid protein, termed PRC5, in pre-mRNA splicing. PRC5 has no homologs in model organisms but was recurrently co-purified with known splicing factors in Trypanosoma brucei. To determine interacting proteins, PRC5 was C-terminally TAP-tagged (we used the PTP tag) and tandem affinity-purified, and co-purifying proteins identified after separation by SDS-PAGE and in-gel trypsin digest by LC/MS/MS. Many of the enriched proteins were known splicing factors, and sucrose gradient sedimentation identified a complex of five subunits, termed PRP19-related complex or PRC.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Trypanosoma Brucei

TISSUE(S): Cell Suspension Culture

SUBMITTER: Arthur Gunzl

LAB HEAD: Arthur Gunzl

PROVIDER: PXD026239 | Pride | 2021-12-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	EOT15-2543_SHPark_r_42663_9.raw	Raw
	EOT15-2544_SHPark_r_42663_8.raw	Raw
	EOT15-2545_SHPark_r_42663_7.raw	Raw
	EOT15-2546_SHPark_r_42663_2.raw	Raw
	EOT15-2547_SHPark_r_42663_1.raw	Raw

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Publications

A distinct complex of PRP19-related and trypanosomatid-specific proteins is required for pre-mRNA splicing in trypanosomes.

Srivastava Ankita A Ambrósio Daniela L DL Tasak Monika M Gosavi Ujwala U Günzl Arthur A

Nucleic acids research 20211201 22

The pre-mRNA splicing factor PRP19 is recruited into the spliceosome after forming the PRP19/CDC5L complex in humans and the Nineteen complex in yeast. Additionally, 'PRP19-related' proteins enter the spliceosome individually or in pre-assemblies that differ in these systems. The protistan family Trypanosomatidae, which harbors parasites such as Trypanosoma brucei, diverged early during evolution from opisthokonts. While introns are rare in these organisms, spliced leader trans splicing is an ob ...[more]

PMID: 34850936

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