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FLAG-PHF3 interactome in HEK293 cells

ABSTRACT: FLAG-hPHF3 was transiently expressed in human HEK293 cells, followed by anti-FLAG immunoprecipitation, on-bead digest and NanoLC-MS analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Richard Imre

LAB HEAD: Dea Slade

PROVIDER: PXD026292 | Pride | 2022-02-17

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20140507_QEx3_RSLC4_Bruno_Slade_MPFL_FLAG_IP1_contr1.msf	Msf
	20140507_QEx3_RSLC4_Bruno_Slade_MPFL_set20_insol_try_pool_5proc_LG_contr1_HeLaMeth.raw	Raw
	20140507_QEx3_RSLC4_Bruno_Slade_MPFL_set20_insol_try_pool_5proc_LG_contr1_onBeadMeth.raw	Raw
	20140509_QEx3_RSLC4_Bruno_Slade_MPFL_FLAG_IP1_sample1.msf	Msf
	20140509_QEx3_RSLC4_Bruno_Slade_MPFL_set20_insol_try_pool_5proc_LG_sample1_HeLaMeth.raw	Raw

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Publications

PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC.

Appel Lisa-Marie LM Franke Vedran V Bruno Melania M Grishkovskaya Irina I Kasiliauskaite Aiste A Kaufmann Tanja T Schoeberl Ursula E UE Puchinger Martin G MG Kostrhon Sebastian S Ebenwaldner Carmen C Sebesta Marek M Beltzung Etienne E Mechtler Karl K Lin Gen G Vlasova Anna A Leeb Martin M Pavri Rushad R Stark Alexander A Akalin Altuna A Stefl Richard R Bernecky Carrie C Djinovic-Carugo Kristina K Slade Dea D

Nature communications 20211019 1

The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) is a regulatory hub for transcription and RNA processing. Here, we identify PHD-finger protein 3 (PHF3) as a regulator of transcription and mRNA stability that docks onto Pol II CTD through its SPOC domain. We characterize SPOC as a CTD reader domain that preferentially binds two phosphorylated Serine-2 marks in adjacent CTD repeats. PHF3 drives liquid-liquid phase separation of phosphorylated Pol II, colocalizes wi ...[more]

PMID: 34667177

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