Dataset Information

Silica associated proteomes of three diatoms reveal conservation of sequence motifs, proteins highly conserved among diatoms, and proteins targeted to specific silica structures

ABSTRACT: Proteins associated with diatom silica are likely involved in the biogenesis of the complex, species-specific morphologies of the biomineral, but only very few such proteins have been identified. In this project we extracted and identified proteins from three related, but morphologically distinct, species of centric diatoms: Thalassiosira pseudonana, Thalassiosira oceanica and Cyclotella cryptica.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive HF

ORGANISM(S): Cyclotella Cryptica Thalassiosira Pseudonana Ccmp1335 Thalassiosira Cf. Oceanica

SUBMITTER: Marc Gentzel

LAB HEAD: Nils Kröger

PROVIDER: PXD026496 | Pride | 2022-05-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20150722_26_cc_tryp_HF_4A.raw	Raw
	20150722_26_cc_tryp_HF_4A_VelosIT_F007658.dat	Other
	20150722_28_cc_chym_HF_4B.raw	Raw
	20150722_28_cc_chym_HF_4B_LR_F007783.dat	Other
	20150722_30_cc_aspn_HF_4C.raw	Raw

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Project description:The rapid growth of diatoms makes them one of the most pervasive and productive types of plankton in the world's ocean, but the physiological basis for their high growth rates remains poorly understood. Here, we evaluate the factors that elevate diatom growth rates, relative to other plankton, using a steady-state metabolic flux model that computes the photosynthetic C source from intracellular light attenuation and the carbon cost of growth from empirical cell C quotas, across a wide range of cell sizes. For both diatoms and other phytoplankton, growth rates decline with increased cell volume, consistent with observations, because the C cost of division increases with size faster than photosynthesis. However, the model predicts overall higher growth rates for diatoms due to reduced C requirements and the low energetic cost of Si deposition. The C savings from the silica frustule are supported by metatranscriptomic data from Tara Oceans, which show that the abundance of transcripts for cytoskeleton components in diatoms is lower than in other phytoplankton. Our results highlight the importance of understanding the origins of phylogenetic differences in cellular C quotas, and suggest that the evolution of silica frustules may play a critical role in the global dominance of marine diatoms. IMPORTANCE This study addresses a longstanding issue regarding diatoms, namely, their fast growth. Diatoms, which broadly are phytoplankton with silica frustules, are the world's most productive microorganisms and dominate in polar and upwelling regions. Their dominance is largely supported by their high growth rate, but the physiological reasoning behind that characteristic has been obscure. In this study, we combine a quantitative model and metatranscriptomic approaches and show that diatoms' low carbon requirements and low energy costs for silica frustule production are the key factors supporting their fast growth. Our study suggests that the effective use of energy-efficient silica as a cellular structure, instead of carbon, enables diatoms to be the most productive organisms in the global ocean.

Dataset Information

Silica associated proteomes of three diatoms reveal conservation of sequence motifs, proteins highly conserved among diatoms, and proteins targeted to specific silica structures

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