Proteomics

Dataset Information

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M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity


ABSTRACT: Genome organization is driven by forces affecting transcriptional state, but the relationship between transcription and genome architecture remains unclear. Here, we identified the Drosophila transcription factor Motif 1 Binding Protein (M1BP) in physical association with the gypsy chromatin insulator core complex, including the universal insulator protein CP190. M1BP is required for enhancer-blocking and barrier activities of the gypsy insulator as well as its proper nuclear localization. Genome-wide, M1BP specifically colocalizes with CP190 at Motif 1-containing promoters, which are enriched at topologically associating domain (TAD) borders. M1BP facilitates CP190 chromatin binding at many shared sites and vice versa. Both factors promote Motif 1-dependent gene expression and transcription near TAD borders genome-wide. Finally, loss of M1BP reduces chromatin accessibility and increases both inter- and intra-TAD local genome compaction. Our results reveal physical and functional interaction between CP190 and M1BP to activate transcription at TAD borders and mediate chromatin insulator-dependent genome organization.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo

SUBMITTER: shue chen  

LAB HEAD: Elissa Lei

PROVIDER: PXD026497 | Pride | 2021-06-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
allSpectra.HCD.ITMS.iso_0.apl Other
allSpectra.HCD.ITMS.iso_1.apl Other
allSpectra.HCD.ITMS.iso_10.apl Other
allSpectra.HCD.ITMS.iso_100.apl Other
allSpectra.HCD.ITMS.iso_101.apl Other
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Publications

M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity.

Bag Indira I   Chen Shue S   Rosin Leah F LF   Chen Yang Y   Liu Chen-Yu CY   Yu Guo-Yun GY   Lei Elissa P EP   Lei Elissa P EP  

Nature communications 20210707 1


Genome organization is driven by forces affecting transcriptional state, but the relationship between transcription and genome architecture remains unclear. Here, we identified the Drosophila transcription factor Motif 1 Binding Protein (M1BP) in physical association with the gypsy chromatin insulator core complex, including the universal insulator protein CP190. M1BP is required for enhancer-blocking and barrier activities of the gypsy insulator as well as its proper nuclear localization. Genom  ...[more]

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