Proteomics

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Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila


ABSTRACT: We describe a drug-based, genetic platform consisting of four selection and two counterselection markers eliminating the need to screen for transgenic progeny. These markers work reliably individually, or in combination, to produce specific genetic outcomes.We perform immunoprecipitation followed by proteomic analysis on one tagged BAC line demonstrating our platform’s applicability to biological discovery.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Head

SUBMITTER: Antrix Jain  

LAB HEAD: Koen J.T. Venken

PROVIDER: PXD026579 | Pride | 2022-02-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
45274_1_00F_fly_ctrl_rep1.msf Msf
45274_1_00F_fly_ctrl_rep1.raw Raw
45275_1_00F_fly_ctrl_rep2.msf Msf
45275_1_00F_fly_ctrl_rep2.raw Raw
45276_1_00F_fly_Csp_rep1.msf Msf
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Publications

Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.

Matinyan Nick N   Karkhanis Mansi S MS   Gonzalez Yezabel Y   Jain Antrix A   Saltzman Alexander A   Malovannaya Anna A   Sarrion-Perdigones Alejandro A   Dierick Herman A HA   Venken Koen J T KJT  

Cell reports 20210901 11


The power of Drosophila melanogaster as a model system relies on tractable germline genetic manipulations. Despite Drosophila's expansive genetics toolbox, such manipulations are still accomplished one change at a time and depend predominantly on phenotypic screening. We describe a drug-based genetic platform consisting of four selection and two counterselection markers, eliminating the need to screen for modified progeny. These markers work reliably individually or in combination to produce spe  ...[more]

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